Key features and details
- Rabbit polyclonal to Histone H2A-Bbd
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Histone H2A-Bbd antibody
DescriptionRabbit polyclonal to Histone H2A-Bbd
SpecificityKnown to detect the recombinant form of human Histone H2A BBD. However, no reaction has yet been demonstrated against the endogenous protein in histone preparations. We believe that this is because of the low abundance of this variant in such preparations.
Tested applicationsSuitable for: ICC, WBmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab4175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 3 µg/ml. Staining may not be specific for H2A.Bbd (see image).|
|WB||1/500. Detects a band of approximately 12.5 kDa (predicted molecular weight: 12.5 kDa). As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.|
FunctionAtypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis.
Tissue specificityPresent in mature sperm.
Sequence similaritiesBelongs to the histone H2A family.
DomainThe docking domain is responsible for the weaker heterodimerization with H2B.
Cellular localizationNucleus. Chromosome. Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies.
- Information by UniProt
- H2A Barr body deficient antibody
- H2A Barr body-deficient antibody
- H2A histone family member B antibody
CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).
The primary antibody dilution was 1:500 and the secondary was 1:3000.
As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.
ab4175 has been referenced in 3 publications.
- Wu BJ et al. Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development. Genet Mol Res 13:5929-39 (2014). PubMed: 25117351
- Nashun B et al. Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice. Development 137:3785-94 (2010). WB, ICC/IF ; Mouse . PubMed: 20943707
- Lee JH et al. Chromatin analysis of occluded genes. Hum Mol Genet 18:2567-74 (2009). ChIP ; Human . PubMed: 19380460