Recombinant

Recombinant Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)

Overview

  • Product name

    Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216]
  • Description

    Rabbit monoclonal [RM216] to Histone H2A (phospho S1) + Histone H4 (phospho S1)
  • Host species

    Rabbit
  • Specificity

    ab222765 reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H2A (phospho S1).

  • Positive control

    • WB: Acid extract of HeLa cells treated with Nocodazole. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab222765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14, 11 kDa.

Images

  • All lanes : Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765) at 0.5 µg/ml

    Lane 1 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole
    Lane 2 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole

    Predicted band size: 14, 11 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2A (phospho S1) + Histone H4 (phospho S1) (red) using ab222765 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).

  • ab222765 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).

References

ab222765 has not yet been referenced specifically in any publications.

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