RecombinantRabMAb

Recombinant Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)

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ELISA - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
  • Dot Blot - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
  • Peptide Array - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
  • ChIP - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
  • Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17588] to Histone H2A (phospho S129) - BSA and Azide free
  • Suitable for: WB, ChIP, Dot blot, PepArr, ELISA
  • Reacts with: Saccharomyces cerevisiae

Overview

  • Product name

    Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR17588] to Histone H2A (phospho S129) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ChIP, Dot blot, PepArr, ELISAmore details

  • Species reactivity

    Reacts with: Saccharomyces cerevisiae

  • Immunogen

    Synthetic peptide within Saccharomyces cerevisiae Histone H2A aa 100 to the C-terminus (phospho S129). The exact sequence is proprietary.
    Database link: P04912

  • Positive control

    • ChIP: Chromatin prepared from Saccharomyces cerevisiae cells.

  • General notes

    Ab241390 is the carrier-free version of ab181447. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab241390 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17588

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ChIP Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
PepArr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Post-translational modification for Saccharomyces cerevisiae: Phosphorylated to form H2AS128ph (gamma-H2A) in response to DNA double-strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks. Phosphorylation is dependent on the DNA damage checkpoint kinases MEC1/ATR and TEL1/ATM, spreads on either side of a detected DSB site and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Gamma-H2A interacts with ARP4, a shared component of the NuA4 histone acetyltransferase complex and the INO80 and SWR1 chromatin remodeling complexes, and serves to recruit first NuA4, mediating histone H4 acetylation, and subsequently the INO80/SWR1 complexes, facilitating DNA resection, to DSB sites. Gamma-H2A is required for sequestering cohesin around the break site, which is important for efficient post-replicative double-strand break repair by homologous recombination, holding the damaged chromatid close to its undamaged sister template. Gamma-H2A is removed from the DNA prior to the strand invasion-primer extension step of the repair process and subsequently dephosphorylated by PPH3, a component of the histone H2A phosphatase complex (HTP-C). Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. N-acetylated by NAT4. Acetylated by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex, to form H2AK4ac and H2AK7ac. Glutamine methylation at Gln-106 (H2AQ105me) by NOP1 is specifically dedicated to polymerase I. It is present at 35S ribosomal DNA locus and impairs binding of the FACT complex. Sumoylated to from H2AK126su. May lead to transcriptional repression. Post-translational modification for Schizosaccharomyces pombe: Phosphorylated to form H2AS128ph (gamma-H2A) in response to DNA double-strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks. Phosphorylation is dependent on the DNA damage checkpoint kinases rad3/ATR and tel1/ATM, spreads on either side of a detected DSB site and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Gamma-H2A is required for recruiting crb2, a modulator of DNA damage checkpoint signaling, to DSB sites. Gamma-H2A is removed from the DNA prior to the strand invasion-primer extension step of the repair process and subsequently dephosphorylated. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. Acetylated by esa1 to form H2AK4ac and H2AK7ac.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • H2A1 antibody
      • H2A2 antibody
      • Histone H2A-alpha antibody
      • Histone H2A-beta antibody
      • Histone H2A.1 antibody
      • Histone H2A.2 antibody
      • Hta 1 antibody
      • HTA 2 antibody
      • Hta1 antibody
      • Hta1p antibody
      • HTA2 antibody
      • Hta2p antibody
      • SPT 11 antibody
      • SPT11 antibody
      see all

    Images

    • ELISA - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
      ELISA - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)

      Serially diluted ab181447 was bound to immobilised phospho (ab19828) - or control (ab19751) peptides (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181447).
    • Dot Blot - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
      Dot Blot - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)

      Dot blot analysis of Histone H2A (phospho S129) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab181447 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time : 3 minutes

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181447).
    • Peptide Array - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
      Peptide Array - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)

      ab181447 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
      Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
      The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181447).
    • ChIP - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
      ChIP - Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)

      Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were treated with MMS at 2mg/ml for 1 h. Treated and non-treated Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab181447 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181447).
    • Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)
      Anti-Histone H2A (phospho S129) antibody [EPR17588] - BSA and Azide free (ab241390)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab241390? Please let us know so that we can cite the reference in this datasheet.

    ab241390 has not yet been referenced specifically in any publications.

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