Recombinant Anti-Histone H2A (phospho S129) antibody [EPR17588] - ChIP Grade (ab181447)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17588] to Histone H2A (phospho S129) - ChIP Grade
- Suitable for: PepArr, Dot blot, ChIP, WB, ELISA
- Reacts with: Human, Saccharomyces cerevisiae
Related conjugates and formulations
Overview
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Product name
Anti-Histone H2A (phospho S129) antibody [EPR17588] - ChIP Grade
See all Histone H2A primary antibodies -
Description
Rabbit monoclonal [EPR17588] to Histone H2A (phospho S129) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: PepArr, Dot blot, ChIP, WB, ELISAmore details -
Species reactivity
Reacts with: Human, Saccharomyces cerevisiae -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Saccharomyces cerevisiae treated with 0.2% Methyl methanesulfonate for 1 hour lysate. ChIP: Chromatin prepared from Saccharomyces cerevisiae cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17588 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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PepArr |
Use at an assay dependent concentration.
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Dot blot |
1/1000.
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ChIP |
Use 2 µg for 25 µg of chromatin.
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WB |
1/5000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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ELISA |
Use at an assay dependent concentration.
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Notes |
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PepArr
Use at an assay dependent concentration. |
Dot blot
1/1000. |
ChIP
Use 2 µg for 25 µg of chromatin. |
WB
1/5000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H2A family. -
Post-translational
modificationsThe chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3012 Human
- Entrez Gene: 317772 Human
- Entrez Gene: 8335 Human
- Entrez Gene: 8338 Human
- Omim: 142720 Human
- Omim: 602786 Human
- Omim: 602797 Human
- SwissProt: P04908 Human
see all -
Alternative names
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
Images
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Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were treated with MMS at 2mg/ml for 1 h. Treated and non-treated Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab181447 (red), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
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All lanes : Anti-Histone H2A (phospho S129) antibody [EPR17588] - ChIP Grade (ab181447) at 1/5000 dilution
Lane 1 : Saccharomyces cerevisiae treated with 0.2% Methyl methanesulfonate for 1 hour lysate
Lane 2 : Untreated Saccharomyces cerevisiae whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of Histone H2A (phospho S129) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab181447 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time : 3 minutes
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ab181447 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181447 has been referenced in 2 publications.
- Reusswig KU et al. Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions. Nat Commun 13:7014 (2022). PubMed: 36400763
- Minchell NE et al. Cohesin Causes Replicative DNA Damage by Trapping DNA Topological Stress. Mol Cell 78:739-751.e8 (2020). PubMed: 32259483