Recombinant
RabMAb

Recombinant Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)

Overview

  • Product name

    Anti-Histone H2A (phospho T120) antibody [EPR17492]
    See all Histone H2A primary antibodies
  • Description

    Rabbit monoclonal [EPR17492] to Histone H2A (phospho T120)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, IHC-P, WB, PepArrmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Histone H2A aa 100 to the C-terminus (phospho T120). The exact sequence is proprietary.
    Database link: P04908

  • Positive control

    • WB: Core histones purified by acid extraction precipitation from HeLa cells treated with colcemid. IHC-P: Human colon, spleen and gastric tumor tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17492
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Due to high affinity of this antibody, we recommend 1/16000 dilution in IHC application.

WB 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
PepArr Use at an assay dependent concentration.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H2A family.
  • Post-translational
    modifications

    The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • H2a 615 antibody
    • H2A antibody
    • H2A GL101 antibody
    • H2A histone family member A antibody
    • H2A.1 antibody
    • H2A.2 antibody
    • H2A/a antibody
    • H2A/m antibody
    • H2A/O antibody
    • H2A/q antibody
    • H2A1B_HUMAN antibody
    • H2AFA antibody
    • H2AFE antibody
    • H2AFL antibody
    • H2AFM antibody
    • H2AFO antibody
    • H2AFQ antibody
    • HIST1H2AE antibody
    • HIST1H2AJ antibody
    • HIST2H2AA antibody
    • HIST2H2AA3 antibody
    • HIST2H2AB antibody
    • HIST2H2AC antibody
    • Histone 1 H2ae antibody
    • Histone 2 H2aa3 antibody
    • Histone 2 H2ab antibody
    • Histone 2 H2ac antibody
    • Histone H2A type 1 B antibody
    • Histone H2A type 1 C antibody
    • Histone H2A type 1 E antibody
    • Histone H2A type 1 J antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    • MGC74460 antibody
    see all

Images

  • All lanes : Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391) at 1/1000 dilution

    Lane 1 : Core histones purified by acid extraction precipitation from untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cells
    Lane 2 : Core histones purified by acid extraction precipitation from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with colcemid

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 2% BSA/TBST.

    This target is specifically expressed at M phase according to the literature [PMID: 19965387].

  • ab177391 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
     
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
     
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
     

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on human colon [PMID: 19965387].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on germinal center of human spleen [PMID: 19965387].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human gastric tumor tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on tumor cells of human gastric tumor [PMID: 19965387].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Direct ELISA analysis using ab177391 (0 to 1000 ng/ml) in peptide ELISA with H2A T120 phospho peptide, H2A unmodified peptide, H2A.XT120 phospho peptide and H2A.X unmodified peptide as antigens (at 1000 ng/ml concentration). An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as secondary antibody.

References

ab177391 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Nothoscordum Cell (Root cell)
Permeabilization
No
Specification
Root cell
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Mrs. MARIELA SADER

Verified customer

Submitted Jun 05 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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