Recombinant Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)
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Overview
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Product name
Anti-Histone H2A (phospho T120) antibody [EPR17492]
See all Histone H2A primary antibodies -
Description
Rabbit monoclonal [EPR17492] to Histone H2A (phospho T120) -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IHC-P, WB, PepArrmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Histone H2A aa 100 to the C-terminus (phospho T120). The exact sequence is proprietary.
Database link: P04908 -
Positive control
- WB: Core histones purified by acid extraction precipitation from HeLa cells treated with colcemid. IHC-P: Human colon, spleen and gastric tumor tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17492 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab177391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Due to high affinity of this antibody, we recommend 1/16000 dilution in IHC application. |
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| WB | 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). | |
| PepArr | Use at an assay dependent concentration. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H2A family. -
Post-translational
modificationsThe chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3012 Human
- Entrez Gene: 317772 Human
- Entrez Gene: 8335 Human
- Entrez Gene: 8338 Human
- Omim: 142720 Human
- Omim: 602786 Human
- Omim: 602797 Human
- SwissProt: P04908 Human
see all -
Alternative names
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
Images
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![Western blot - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)](https://www.abcam.com/ps/products/177/ab177391/Images/ab177391-276154-anti-histone-h2a-phospho-t120-antibody-epr17492-western-blot.jpg)
Western blot - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)All lanes : Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391) at 1/1000 dilution
Lane 1 : Core histones purified by acid extraction precipitation from untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cells
Lane 2 : Core histones purified by acid extraction precipitation from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with colcemid
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 2% BSA/TBST.
This target is specifically expressed at M phase according to the literature [PMID: 19965387].
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![Peptide Array - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)](https://www.abcam.com/ps/products/177/ab177391/Images/ab177391-277294-anti-histone-h2a-phospho-t120-antibody-epr17492-peptide-array.jpg)
Peptide Array - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)ab177391 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)](https://www.abcam.com/ps/products/177/ab177391/Images/ab177391-276152-anti-histone-h2a-phospho-t120-antibody-epr17492-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on human colon [PMID: 19965387].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)](https://www.abcam.com/ps/products/177/ab177391/Images/ab177391-276151-anti-histone-h2a-phospho-t120-antibody-epr17492-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on germinal center of human spleen [PMID: 19965387].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)](https://www.abcam.com/ps/products/177/ab177391/Images/ab177391-276150-anti-histone-h2a-phospho-t120-antibody-epr17492-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)Immunohistochemical analysis of paraffin-embedded human gastric tumor tissue labeling Histone H2A (phospho T120) with ab177391 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadically nuclear staining on tumor cells of human gastric tumor [PMID: 19965387].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![ELISA - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)](https://www.abcam.com/ps/products/177/ab177391/Images/ab177391-286779-anti-histone-h2a-phospho-t120-antibody-epr17492-elisa.jpg)
ELISA - Anti-Histone H2A (phospho T120) antibody [EPR17492] (ab177391)Direct ELISA analysis using ab177391 (0 to 1000 ng/ml) in peptide ELISA with H2A T120 phospho peptide, H2A unmodified peptide, H2A.XT120 phospho peptide and H2A.X unmodified peptide as antigens (at 1000 ng/ml concentration). An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as secondary antibody.
Datasheets and documents
References
ab177391 has not yet been referenced specifically in any publications.
