Overview

  • Product name

    Anti-Histone H2A (yeast) (phospho S129) antibody
    See all Histone H2A primary antibodies
  • Description

    Rabbit polyclonal to Histone H2A (phospho S129)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Schizosaccharomyces pombe
    Predicted to work with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Schizosaccharomyces pombe Histone H2A aa 100 to the C-terminus (C terminal) (phospho S129) conjugated to keyhole limpet haemocyanin.
    Database link: P04910
    (Peptide available as ab17576)

  • Positive control

    • S. pombe lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15.5 kDa (predicted molecular weight: 15.5 kDa).

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H2A family.
  • Post-translational
    modifications

    The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

    • Alternative names

      • H2a 615 antibody
      • H2A antibody
      • H2A GL101 antibody
      • H2A histone family member A antibody
      • H2A.1 antibody
      • H2A.2 antibody
      • H2A/a antibody
      • H2A/m antibody
      • H2A/O antibody
      • H2A/q antibody
      • H2A1B_HUMAN antibody
      • H2AFA antibody
      • H2AFE antibody
      • H2AFL antibody
      • H2AFM antibody
      • H2AFO antibody
      • H2AFQ antibody
      • HIST1H2AE antibody
      • HIST1H2AJ antibody
      • HIST2H2AA antibody
      • HIST2H2AA3 antibody
      • HIST2H2AB antibody
      • HIST2H2AC antibody
      • Histone 1 H2ae antibody
      • Histone 2 H2aa3 antibody
      • Histone 2 H2ab antibody
      • Histone 2 H2ac antibody
      • Histone H2A type 1 B antibody
      • Histone H2A type 1 C antibody
      • Histone H2A type 1 E antibody
      • Histone H2A type 1 J antibody
      • Histone H2A type 1-B/E antibody
      • Histone H2A.2 antibody
      • Histone H2A/a antibody
      • Histone H2A/m antibody
      • MGC74460 antibody
      see all

    Images

    • ab17353 recognised a band of the predicted size in S. pombe lysate (lane 1). This band was blocked by the immunising peptide (lane 2) but not by the unmodified version of the immunising peptide (lane 3). This suggests specificity for the modified residue.

    References

    This product has been referenced in:

    • Jablonowski CM  et al. Termination of Replication Stress Signaling via Concerted Action of the Slx4 Scaffold and the PP4 Phosphatase. Genetics 201:937-49 (2015). Read more (PubMed: 26362319) »
    • Tapia-Alveal C  et al. H2A.Z-dependent regulation of cohesin dynamics on chromosome arms. Mol Cell Biol 34:2092-104 (2014). Read more (PubMed: 24687850) »
    See all 5 Publications for this product

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