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Herewith the sequence of Arabidopsis gamma H2AX. I have highlighted the region others have used to make their custom antibodies.
It would be good to know which of your vast arrays of custom abs would be best to use. I’m primarily interested in immunocytochemistry in the first instance!
Thanks so much for any help you can give,
Asked on Jul 02 2012
Thank you for contacting us and your interest in our products.
I have had a look through the antibodies we have directed against the H2A.X. Unfortunately, most are not likely to react with the target you are hoping to detect in Arabidopsis. This is due to the low sequence homology of the immunogen used to raise the antibody compared to the sequence you have provided for me.
The antibody ab11174 is raised against a peptide taken around thephospho-S139 of human H2A.X. This region again shares very low homology with the sequence you are interested in and I would therefore suggest it would be unlikely to work (please refer to the sequence alignment attached).
The antibodywe have which I think would be most likely to work is ab124781. This is a rabbit monoclonal raised against the region highlighted in yellow of the attachment. As you can see, this is still not a very good match (11 of the 17 residues match) but as it is a rabbit monoclonal, if it does bind the region of similarity it will work and the reactivity between lots is likely to be consistent. As we have not tried this ourselves we can of course not guarantee that it would work.
I hope this information has been of help. If you require any further information, please do not hesitate to ask.
Answered on Jul 02 2012