Recombinant

Recombinant Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)

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Overview

  • Product name

    Anti-Histone H2A.Z (acetyl K4) antibody [RM221]
    See all Histone H2A.Z primary antibodies

  • Description

    Rabbit monoclonal [RM221] to Histone H2A.Z (acetyl K4)

  • Host species

    Rabbit

  • Specificity

    ab214725 reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species

  • Immunogen

    Synthetic peptide corresponding to Human Histone H2A.Z (acetyl K4).
    Database link: P0C0S5

  • Positive control

    • HeLa cells extracts, treated with sodium butyrate.

Properties

Applications

Our Abpromise guarantee covers the use of ab214725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.

Target

  • Function

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

  • Sequence similarities

    Belongs to the histone H2A family.

  • Post-translational
    modifications

    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.

  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    Images

    • Western blot - Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
      Western blot - Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
      All lanes : Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725) at 0.5 µg/ml

      Lane 1 : HeLa cell extracts, treated with sodium butyrate
      Lane 2 : HeLa cell extracts, untreated

      Predicted band size: 14 kDa

    References

    ab214725 has not yet been referenced specifically in any publications.

    Publishing research using ab214725? Please let us know so that we can cite the reference in this datasheet.

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