Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM221] to Histone H2A.Z (acetyl K4)
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Histone H2A.Z (acetyl K4) antibody [RM221]
See all Histone H2A.Z primary antibodies
DescriptionRabbit monoclonal [RM221] to Histone H2A.Z (acetyl K4)
Specificityab214725 reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Synthetic peptide corresponding to Human Histone H2A.Z (acetyl K4).
Database link: P0C0S5
- HeLa cells extracts, treated with sodium butyrate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.|
FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Sequence similaritiesBelongs to the histone H2A family.
modificationsMonoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
ab214725 has not yet been referenced specifically in any publications.