Recombinant

Recombinant Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)

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Western blot - Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
  • Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM221] to Histone H2A.Z (acetyl K4)
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H2A.Z (acetyl K4) antibody [RM221]
    See all Histone H2A.Z primary antibodies

  • Description

    Rabbit monoclonal [RM221] to Histone H2A.Z (acetyl K4)

  • Host species

    Rabbit

  • Specificity

    ab214725 reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species

  • Immunogen

    Synthetic peptide corresponding to Human Histone H2A.Z (acetyl K4).
    Database link: P0C0S5

  • Positive control

    • HeLa cells extracts, treated with sodium butyrate.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    RM221

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.

Target

  • Function

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

  • Sequence similarities

    Belongs to the histone H2A family.

  • Post-translational
    modifications

    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.

  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    Images

    • Western blot - Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
      Western blot - Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
      All lanes : Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725) at 0.5 µg/ml

      Lane 1 : HeLa cell extracts, treated with sodium butyrate
      Lane 2 : HeLa cell extracts, untreated

      Predicted band size: 14 kDa

    • Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
      Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)

    References (0)

    Publishing research using ab214725? Please let us know so that we can cite the reference in this datasheet.

    ab214725 has not yet been referenced specifically in any publications.

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