Recombinant

Recombinant Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)

Overview

  • Product name

    Anti-Histone H2A.Z (acetyl K7) antibody [RM222]
    See all Histone H2A.Z primary antibodies
  • Description

    Rabbit monoclonal [RM222] to Histone H2A.Z (acetyl K7)
  • Host species

    Rabbit
  • Specificity

    ab214730 reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.
  • Tested applications

    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human Histone H2A.Z (acetyl K7).
    Database link: P0C0S5

  • Positive control

    • Acid extracts from HeLa cells treated with sodium butyrate.

Properties

Applications

Our Abpromise guarantee covers the use of ab214730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.2 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.

Target

  • Function

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • Sequence similarities

    Belongs to the histone H2A family.
  • Post-translational
    modifications

    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • H2A histone family member Z antibody
    • H2A.z antibody
    • H2A/z antibody
    • H2afz antibody
    • H2AZ antibody
    • H2AZ_HUMAN antibody
    • Histone H2A.Z antibody
    • MGC117173 antibody
    see all

Images

  • All lanes : Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730) at 0.5 µg/ml

    Lane 1 : acid extracts from HeLa cells treated with sodium butyrate
    Lane 2 : acid extracts from HeLa cells, untreated

    Predicted band size: 14 kDa

  • ELISA analysis. ab214730 specifically reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.

  • Immunocytochemical staining of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate, stained for Histone H2A.Z (acetyl K7) using ab214730 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

References

This product has been referenced in:

  • Wang G  et al. Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica. AMB Express 7:94 (2017). WB . Read more (PubMed: 28497289) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Arabidopsis thaliana Tissue lysate - nuclear (Seedling)
Gel Running Conditions
Reduced Denaturing (15% SDS PAGE)
Loading amount
0.5 µg
Specification
Seedling
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Yogendra Bordiya

Verified customer

Submitted Sep 08 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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