Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM222] to Histone H2A.Z (acetyl K7)
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Human
Product nameAnti-Histone H2A.Z (acetyl K7) antibody [RM222]
See all Histone H2A.Z primary antibodies
DescriptionRabbit monoclonal [RM222] to Histone H2A.Z (acetyl K7)
Specificityab214730 reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.
Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Synthetic peptide corresponding to Human Histone H2A.Z (acetyl K7).
Database link: P0C0S5
- Acid extracts from HeLa cells treated with sodium butyrate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA, 48% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.2 - 1 µg/ml.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.|
FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Sequence similaritiesBelongs to the histone H2A family.
modificationsMonoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
All lanes : Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730) at 0.5 µg/ml
Lane 1 : acid extracts from HeLa cells treated with sodium butyrate
Lane 2 : acid extracts from HeLa cells, untreated
Predicted band size: 14 kDa
ELISA analysis. ab214730 specifically reacts to Histone H2A.Z acetylated
at Lysine 7 (K7ac). No cross reactivity with non-modified
Lysine 7 or other acetylated Lysines in histone H2A.
Immunocytochemical staining of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate, stained for Histone H2A.Z (acetyl K7) using ab214730 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ab214730 has been referenced in 1 publication.
- Wang G et al. Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica. AMB Express 7:94 (2017). WB . PubMed: 28497289