• Product name
    Anti-Histone H2A.Z antibody
    See all Histone H2A.Z primary antibodies
  • Description
    Sheep polyclonal to Histone H2A.Z
  • Host species
  • Tested applications
    Suitable for: ICC/IF, ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Chicken Histone H2A.Z aa 1-17 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).




Our Abpromise guarantee covers the use of ab18263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).


  • Function
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
  • Sequence similarities
    Belongs to the histone H2A family.
  • Post-translational
    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
    Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
    Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
    Not phosphorylated.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H2A histone family member Z antibody
    • H2A.z antibody
    • H2A/z antibody
    • H2afz antibody
    • H2AZ antibody
    • H2AZ_HUMAN antibody
    • Histone H2A.Z antibody
    • MGC117173 antibody
    see all


  • All lanes : Anti-Histone H2A.Z antibody (ab18263)

    Lanes 1 & 4 : Butyrate-treated HeLa histones
    Lanes 2 & 5 : 15-day embryo chicken erythrocyte histones
    Lanes 3 & 6 : Recombinant H2A.Z

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

  • All lanes : Anti-Histone H2A.Z antibody (ab18263)

    Lanes 1 & 6 : Butyrate-treated HeLa histones
    Lanes 2 & 7 : Ix3 extract
    Lanes 3 & 8 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.

    Lanes 4 & 9 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.

    Lanes 5 & 10 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

    Nucleosomes from 10-day chicken brain tissue were used in ChIP experiments. An acetic acid / urea / Triton gel was run and the corresponding western blot using ab18263 was used to compare proteins in the Bound chromatin fraction with those of the Input and Unbound fractions. In lane 10 there is a strong band corresponding to a hyper-acetylated H2A.Z.


This product has been referenced in:
  • Veith N  et al. Mechanisms underlying epigenetic and transcriptional heterogeneity in Chinese hamster ovary (CHO) cell lines. BMC Biotechnol 16:6 (2016). ChIP . Read more (PubMed: 26800878) »
  • Valdes-Quezada C  et al. CTCF demarcates chicken embryonic a-globin gene autonomous silencing and contributes to adult stage-specific gene expression. Epigenetics 8:827-38 (2013). ChIP . Read more (PubMed: 23880533) »

See all 9 Publications for this product

Customer reviews and Q&As

The amino acid sequence of the immunogen that was used to generate the Anti-Histone H2A.Z antibody ab18263 is 100% conserved in the sequence you sent. We expect the antibody will cross-react with Strongylocentrotus purpuratus but we do not guarantee th...

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Thank you for contacting us. Unfortunately, these H2A.Z- and H2A antibodies have not been tested in ELISA or dot blots and we therefore cannot guarantee them to work in these applications. Ab18262 and ab18263 have been tested in Western blot aga...

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Thank you for your enquiry. I have performed an alignment with the sequences of each of the immunogens of ab4174 and ab18263 against Drosophila sequence NP_524519. Once again the results can be viewed below. http://www.ebi.ac.uk/cgi-bin/clustalw...

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Thank you for your enquiry. US Customs are currently preventing all antibodies raised in sheep, goat, donkey, horse, pig and chicken from entering into the States unless you have a USDA import permit in place for these items. You can view inform...

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