Product nameAnti-Histone H2A.Z antibody [EPR6172]
See all Histone H2A.Z primary antibodies
DescriptionRabbit monoclonal [EPR6172] to Histone H2A.Z
SpecificityThis antibody is known to detect both isoforms of H2A.Z (H2A.Z.1 and H2A.Z.2).
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Histone H2A.Z aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
- C6, Raw 264.7, PC-12, NIH/3T3, HeLa, HepG2, and Human thymus lysates , Human breast tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 14 kDa.|
|IHC-P||1/250 - 1/500. antigen retrieval is recommended.|
FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Sequence similaritiesBelongs to the histone H2A family.
modificationsMonoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
All lanes : Anti-Histone H2A.Z antibody [EPR6172] (ab124793) at 1/1000 dilution
Lane 1 : C6 lysates
Lane 2 : Raw 264.7 lysates
Lane 3 : PC-12 lysates
Lane 4 : NIH/3T3 lysates
Lane 5 : HeLa lysates
Lane 6 : HepG2 lysates
Lane 7 : Human thymus lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDa
ab124793, at a 1/250 dilution, staining Histone H2A.Z in paraffin embedded Human breast tissue by Immunohistochemistry.
ab124793 has not yet been referenced specifically in any publications.