Product nameAnti-Histone H2A.Z antibody [IC10]
See all Histone H2A.Z primary antibodies
DescriptionRat monoclonal [IC10] to Histone H2A.Z
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Orangutan
Synthetic peptide corresponding to Histone H2A.Z.
- This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Our Abpromise guarantee covers the use of ab4462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 13.5 kDa).|
FunctionVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Sequence similaritiesBelongs to the histone H2A family.
modificationsMonoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
All lanes : Anti-Histone H2A.Z antibody [IC10] (ab4462) at 10 µg/ml
Lane 1 : Histone H2A.Z recombinant protein
Lane 2 : Histone H2A recombinant protein
Lysates/proteins at 0.5 µg per lane.
All lanes : Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 13.5 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab4462 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
ab4462 has not yet been referenced specifically in any publications.