Overview

  • Product name
    Anti-Histone H2A.Z antibody [IC10]
    See all Histone H2A.Z primary antibodies
  • Description
    Rat monoclonal [IC10] to Histone H2A.Z
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human, Recombinant fragment
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Histone H2A.Z.

  • Positive control
    • This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    IC10
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 10 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 13.5 kDa).
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
    • Sequence similarities
      Belongs to the histone H2A family.
    • Post-translational
      modifications
      Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.
    • Cellular localization
      Nucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    Images

    • All lanes : Anti-Histone H2A.Z antibody [IC10] (ab4462) at 10 µg/ml

      Lane 1 : Histone H2A.Z recombinant protein
      Lane 2 : Histone H2A recombinant protein

      Lysates/proteins at 0.5 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 13.5 kDa
      Observed band size: 15 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes
    • ab4462 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP.  For further experimental details please refer to abreview.

      See Abreview

    References

    ab4462 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting Abcam.

    All of our products are for research purposes only and are not certified for clinical research applications.

    I am sorry that I could not be more helpful in this matter.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreatic cancer)
    Specification
    Pancreatic cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

    Mr. Antibody Solutions

    Verified customer

    Submitted Mar 11 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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