- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Histone H2B (acetyl K12) antibody [EP858Y]
See all Histone H2B primary antibodies -
DescriptionRabbit monoclonal [EP858Y] to Histone H2B (acetyl K12)
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K12). The exact sequence is proprietary.
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Positive control
- HeLa cells, Human colon carcinoma. IHC-P: Human normal colon FFPE tissue sections.
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General notes
This antibody only detects Histone H2B acetylated on Lysine 12.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
PurityTissue culture supernatant
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Primary antibody notesThis antibody only detects Histone H2B acetylated on Lysine 12.
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ClonalityMonoclonal
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Clone numberEP858Y
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab40883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa). | |
| ICC/IF | 1/250 - 1/500. |
Target
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FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Sequence similaritiesBelongs to the histone H2B family.
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Post-translational
modificationsMonoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. -
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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Database links
- Entrez Gene: 440689 Human
- Entrez Gene: 54145 Human
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
- SwissProt: P33778 Human
- SwissProt: P57053 Human
- SwissProt: P58876 Human
see all -
Alternative names
- H2B 1A antibody
- H2B antibody
- H2B histone family antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)](https://www.abcam.com/ps/products/40/ab40883/Images/ab40883-218250-ab408831in200edit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)IHC image of ab40883 staining Histone H2B (acetyl K12) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40883, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)](https://www.abcam.com/ps/products/40/ab40883/Images/ab40883a.gif)
Western blot - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883) at 1/2000 dilution + C6+TSA cell lysate at 10 µg
Predicted band size: 14 kDa
Observed band size: 15 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)](https://www.abcam.com/ps/products/40/ab40883/Images/ab40883b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B ab40883 at a 1:250 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)](https://www.abcam.com/ps/products/40/ab40883/Images/ab40883c.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)ab40883 (1:250) staining of HeLa cells by immunofluorescence. -
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)](https://www.abcam.com/ps/products/40/ab40883/Images/Histone-H2B-Primary-antibodies-ab40883-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)Image courtesy of an abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab40883 (1/500) staining Histone H2B (acetyl K12) in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised using 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
References
ab40883 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"