Overview

  • Product name
    Anti-Histone H2B (acetyl K12) antibody [EP858Y]
    See all Histone H2B primary antibodies
  • Description
    Rabbit monoclonal [EP858Y] to Histone H2B (acetyl K12)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K12). The exact sequence is proprietary.

  • Positive control
    • HeLa cells, Human colon carcinoma. IHC-P: Human normal colon FFPE tissue sections.
  • General notes

    This antibody only detects Histone H2B acetylated on Lysine 12.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 99% Tissue culture supernatant, 0.1% BSA
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    This antibody only detects Histone H2B acetylated on Lysine 12.
  • Clonality
    Monoclonal
  • Clone number
    EP858Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • H2B GL105 antibody
      • H2B histone family member O antibody
      • H2B histone family member S antibody
      • H2B.1 antibody
      • H2B.1 B antibody
      • H2B.b antibody
      • H2B.c antibody
      • H2B.d antibody
      • H2B.e antibody
      • H2B.f antibody
      • H2B.j antibody
      • H2B.q antibody
      • H2B/b antibody
      • H2B/c antibody
      • H2B/d antibody
      • H2B/e antibody
      • H2B/f antibody
      • H2B/j antibody
      • H2B/o antibody
      • H2B/q antibody
      • H2BFB antibody
      • H2BFC antibody
      • H2BFD antibody
      • H2BFE antibody
      • H2BFF antibody
      • H2BFJ antibody
      • H2BFO antibody
      • H2BFQ antibody
      • H2BFS antibody
      • HIRIP2 antibody
      • HIST1H2BB antibody
      • HIST1H2BD antibody
      • HIST1H2BH antibody
      • HIST1H2BL antibody
      • HIST1H2BM antibody
      • HIST1H2BN antibody
      • HIST2H2BE antibody
      • Histone H2B antibody
      • Histone H2B type 1 B antibody
      • Histone H2B type 1 D antibody
      • Histone H2B type 1 H antibody
      • Histone H2B type 1 L antibody
      • Histone H2B type 1 M antibody
      • Histone H2B type 1 N antibody
      • Histone H2B type 2 E antibody
      • histone protein antibody
      see all

    Images

    • IHC image of ab40883 staining Histone H2B (acetyl K12) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40883, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883) at 1/2000 dilution + C6+TSA cell lysate at 10 µg

      Predicted band size: 14 kDa
      Observed band size: 15 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B ab40883 at a 1:250 dilution.
    • ab40883 (1:250) staining of HeLa cells by immunofluorescence.
    • ab40883 (1/500) staining Histone H2B (acetyl K12) in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised using 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    References

    This product has been referenced in:
    • Brocks D  et al. DNMT and HDAC inhibitors induce cryptic transcription start sites encoded in long terminal repeats. Nat Genet 49:1052-1060 (2017). Read more (PubMed: 28604729) »
    • Gardner NM  et al. Elevated Nuclear and Cytoplasmic FTY720-Phosphate in Mouse Embryonic Fibroblasts Suggests the Potential for Multiple Mechanisms in FTY720-Induced Neural Tube Defects. Toxicol Sci 150:161-8 (2016). Read more (PubMed: 26719367) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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