Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)


  • Product name
    Anti-Histone H2B (acetyl K12) antibody [EP858Y]
    See all Histone H2B primary antibodies
  • Description
    Rabbit monoclonal [EP858Y] to Histone H2B (acetyl K12)
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K12). The exact sequence is proprietary.

  • Positive control
    • HeLa cells, Human colon carcinoma. IHC-P: Human normal colon FFPE tissue sections.
  • General notes

    This antibody only detects Histone H2B acetylated on Lysine 12.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 99% Tissue culture supernatant, 0.1% BSA
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    This antibody only detects Histone H2B acetylated on Lysine 12.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab40883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • Sequence similarities
      Belongs to the histone H2B family.
    • Post-translational
      Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
      Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
    • Cellular localization
      Nucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • H2B 1A antibody
      • H2B antibody
      • H2B histone family antibody
      • H2B2f antibody
      • H2B2F_HUMAN antibody
      • H2Ba antibody
      • H2Bf antibody
      • HIST2H2BF antibody
      • histone H2B antibody
      • histone H2B type 1 antibody
      • Histone H2B type 2-F antibody
      • MGC131639 antibody
      see all


    • IHC image of ab40883 staining Histone H2B (acetyl K12) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40883, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883) at 1/2000 dilution + C6+TSA cell lysate at 10 µg

      Predicted band size: 14 kDa
      Observed band size: 15 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B ab40883 at a 1:250 dilution.
    • ab40883 (1:250) staining of HeLa cells by immunofluorescence.
    • ab40883 (1/500) staining Histone H2B (acetyl K12) in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised using 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview


    ab40883 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)
    Yes - 0.5% Triton X100

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 15 2011


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