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Epigenetics and Nuclear Signaling Histones H2B Acetylated
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RabMAb

Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
  • Western blot - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)

Key features and details

  • Rabbit monoclonal [EP858Y] to Histone H2B (acetyl K12)
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H2B (acetyl K12) antibody [EP858Y]
    See all Histone H2B primary antibodies
  • Description

    Rabbit monoclonal [EP858Y] to Histone H2B (acetyl K12)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K12). The exact sequence is proprietary.

  • Positive control

    • HeLa cells, Human colon carcinoma. IHC-P: Human normal colon FFPE tissue sections.
  • General notes

    This antibody only detects Histone H2B acetylated on Lysine 12.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 99% Tissue culture supernatant, 0.1% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Primary antibody notes

    This antibody only detects Histone H2B acetylated on Lysine 12.
  • Clonality

    Monoclonal
  • Clone number

    EP858Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H2B
    • Acetylated

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Histone H2B protein (ab92409)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
Application Abreviews Notes
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
ICC/IF (1)
1/250 - 1/500.
Notes
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
    • Cellular localization

      Nuclear
    • Database links

      • Entrez Gene: 337874 Human
      • Entrez Gene: 54145 Human
      • Entrez Gene: 8349 Human
      • SwissProt: O60814 Human
      • SwissProt: P06899 Human
      • SwissProt: P23527 Human
      • SwissProt: P33778 Human
      • SwissProt: P57053 Human
      • SwissProt: P58876 Human
      • SwissProt: P62807 Human
      • SwissProt: Q16778 Human
      • SwissProt: Q5QNW6 Human
      • SwissProt: Q8N257 Human
      • SwissProt: Q93079 Human
      • SwissProt: Q99877 Human
      • SwissProt: Q99879 Human
      • SwissProt: Q99880 Human
      see all
    • Alternative names

      • GL105 antibody
      • H2B GL105 antibody
      • H2B histone family member O antibody
      • H2B histone family member S antibody
      • H2B.1 antibody
      • H2B.1 B antibody
      • H2B.b antibody
      • H2B.c antibody
      • H2B.d antibody
      • H2B.e antibody
      • H2B.f antibody
      • H2B.j antibody
      • H2B.q antibody
      • H2B/b antibody
      • H2B/c antibody
      • H2B/d antibody
      • H2B/e antibody
      • H2B/f antibody
      • H2B/j antibody
      • H2B/o antibody
      • H2B/q antibody
      • H2BFB antibody
      • H2BFC antibody
      • H2BFD antibody
      • H2BFE antibody
      • H2BFF antibody
      • H2BFJ antibody
      • H2BFO antibody
      • H2BFQ antibody
      • H2BFS antibody
      • H2BGL105 antibody
      • H2BQ antibody
      • HIRIP2 antibody
      • HIST1H2BB antibody
      • HIST1H2BD antibody
      • HIST1H2BH antibody
      • HIST1H2BL antibody
      • HIST1H2BM antibody
      • HIST1H2BN antibody
      • HIST2H2BE antibody
      • histone cluster 2 antibody
      • Histone H2B antibody
      • histone H2B GL105 antibody
      • Histone H2B type 1 B antibody
      • Histone H2B type 1 D antibody
      • Histone H2B type 1 H antibody
      • Histone H2B type 1 L antibody
      • Histone H2B type 1 M antibody
      • Histone H2B type 1 N antibody
      • Histone H2B type 2 E antibody
      • Histone H2B.q antibody
      • histone protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)

      IHC image of ab40883 staining Histone H2B (acetyl K12) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40883, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
      Western blot - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
      Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883) at 1/2000 dilution + C6+TSA cell lysate at 10 µg

      Predicted band size: 14 kDa
      Observed band size: 15 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)

      Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B ab40883 at a 1:250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
      Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
      ab40883 (1:250) staining of HeLa cells by immunofluorescence.
    • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
      Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)Image courtesy of an abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
      ab40883 (1/500) staining Histone H2B (acetyl K12) in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised using 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab40883? Please let us know so that we can cite the reference in this datasheet.

    ab40883 has been referenced in 2 publications.

    • Brocks D  et al. DNMT and HDAC inhibitors induce cryptic transcription start sites encoded in long terminal repeats. Nat Genet 49:1052-1060 (2017). PubMed: 28604729
    • Gardner NM  et al. Elevated Nuclear and Cytoplasmic FTY720-Phosphate in Mouse Embryonic Fibroblasts Suggests the Potential for Multiple Mechanisms in FTY720-Induced Neural Tube Defects. Toxicol Sci 150:161-8 (2016). PubMed: 26719367

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2B (acetyl K12) antibody [EP858Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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