Key features and details
- Rabbit polyclonal to Histone H2B (acetyl K15)
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Histone H2B (acetyl K15) antibody
See all Histone H2B primary antibodies
DescriptionRabbit polyclonal to Histone H2B (acetyl K15)
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Histone H2B (acetyl K15). The exact sequence is proprietary.
Database link: P62807
- WB: HeLa, K562 and HEK-293, treated with with 30mM sodium butyrate for 4hr, whole cell lysates. ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4hr). IP: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4hr).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 14 kDa.|
|IP||1/200 - 1/2000.|
|ICC/IF||1/1 - 1/10.|
RelevanceCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
All lanes : Anti-Histone H2B (acetyl K15) antibody (ab240891) at 1/1000 dilution
Lane 1 : Hela (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 4 : Hela whole cell lysate, untreated (-)
Lane 5 : K562 whole cell lysate, untreated (-)
Lane 6 : A549 whole cell lysate, untreated (-)
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 14 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) stained for Histone H2B (acetyl K15) using ab240891 at 1/5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was an Alexa-Fluor®488-conjugated Goat anti-Rabbit IgG(H+L).
Histone H2B (acetyl K215) was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (treated with 30mM sodium butyrate for 4hr) with ab240891 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4hr).
Lane 2: ab240891 IP in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4hr).
Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4hr) 20 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240891 has not yet been referenced specifically in any publications.