Recombinant Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15) - ChIP Grade
- Suitable for: ChIP-sequencing, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade
See all Histone H2B primary antibodies -
Description
Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15) - ChIP Grade -
Host species
Rabbit -
Specificity
This antibody only detects Histone H2B acetylated on Lysine 15. -
Tested applications
Suitable for: ChIP-sequencing, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa whole cell lysate (ab150035) or human breast carcinoma tissue; ChIP-seq: Chromatin prepared from HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP955Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ChIP-sequencing |
Use 4µg for 107 cells.
|
|
WB | (2) |
1/2000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
|
IHC-P | (1) |
1/100 - 1/250.
|
Notes |
---|
ChIP-sequencing
Use 4µg for 107 cells. |
WB
1/2000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa). |
IHC-P
1/100 - 1/250. |
Target
-
Relevance
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- Entrez Gene: 104021 Mouse
- Entrez Gene: 64647 Rat
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
see all -
Alternative names
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
Images
-
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 µg of ab62335 [EP955Y]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be downloaded here. -
All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] - ChIP Grade (ab62335) at 1/2000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with TSA)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
The bottom image shows beta Tubulin as a positive control. -
Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab62335 has been referenced in 4 publications.
- Xia L et al. Modulation of IL-6 Expression by KLF4-Mediated Transactivation and PCAF-Mediated Acetylation in Sublytic C5b-9-Induced Rat Glomerular Mesangial Cells. Front Immunol 12:779667 (2021). PubMed: 35046941
- Lu M et al. ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition. Cell Metab 29:886-900.e5 (2019). PubMed: 30661930
- Dong L et al. Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth. Sci Rep 4:5432 (2014). PubMed: 24961246
- Vlaicu SI et al. Epigenetic modifications induced by RGC-32 in colon cancer. Exp Mol Pathol 88:67-76 (2010). PubMed: 19883641