Product nameAnti-Histone H2B (acetyl K15) antibody [EP955Y]
See all Histone H2B primary antibodies
DescriptionRabbit monoclonal [EP955Y] to Histone H2B (acetyl K15)
SpecificityThis antibody only detects Histone H2B acetylated on Lysine 15.
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K15). The exact sequence is proprietary.
- HeLa whole cell lysate (ab150035) or human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab62335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/500.|
|WB||1/2000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).|
|IHC-P||1/100 - 1/250.|
RelevanceCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335) at 1/2000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with TSA)
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
The bottom image shows beta Tubulin as a positive control.
Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
ab62335 (1/500) staining Histone H2B (acetyl K15) in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.
This product has been referenced in:
- Dong L et al. Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth. Sci Rep 4:5432 (2014). Read more (PubMed: 24961246) »
- Vlaicu SI et al. Epigenetic modifications induced by RGC-32 in colon cancer. Exp Mol Pathol 88:67-76 (2010). Read more (PubMed: 19883641) »