For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    histone-h2b-acetyl-k15-antibody-ep955y-bsa-and-azide-free-ab247351.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Share by email
RecombinantRabMAb

Recombinant Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
  • Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15) - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
Product image
PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
Protein
Product image
Recombinant Human Histone H2B protein (ab92409)

View more associated products

Overview

  • Product name

    Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free
    See all Histone H2B primary antibodies
  • Description

    Rabbit monoclonal [EP955Y] to Histone H2B (acetyl K15) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Histone H2B acetylated on Lysine 15.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247351 is the carrier-free version of ab62335. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247351 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP955Y
  • Isotype

    IgG

Associated products

  • Alternative Versions

    • Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
  • Recombinant Protein

    • Recombinant Human Histone H2B protein (ab92409)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
Notes
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
    • Cellular localization

      Nuclear
    • Database links

      • Entrez Gene: 337874 Human
      • Entrez Gene: 54145 Human
      • Entrez Gene: 8349 Human
      • Entrez Gene: 104021 Mouse
      • Entrez Gene: 64647 Rat
      • SwissProt: O60814 Human
      • SwissProt: P06899 Human
      • SwissProt: P23527 Human
      • SwissProt: P33778 Human
      • SwissProt: P57053 Human
      • SwissProt: P58876 Human
      • SwissProt: P62807 Human
      • SwissProt: Q16778 Human
      • SwissProt: Q5QNW6 Human
      • SwissProt: Q8N257 Human
      • SwissProt: Q93079 Human
      • SwissProt: Q99877 Human
      • SwissProt: Q99879 Human
      • SwissProt: Q99880 Human
      • SwissProt: P10854 Mouse
      • SwissProt: Q64475 Mouse
      • SwissProt: Q00729 Rat
      see all
    • Alternative names

      • GL105 antibody
      • H2B GL105 antibody
      • H2B histone family member O antibody
      • H2B histone family member S antibody
      • H2B.1 antibody
      • H2B.1 B antibody
      • H2B.b antibody
      • H2B.c antibody
      • H2B.d antibody
      • H2B.e antibody
      • H2B.f antibody
      • H2B.j antibody
      • H2B.q antibody
      • H2B/b antibody
      • H2B/c antibody
      • H2B/d antibody
      • H2B/e antibody
      • H2B/f antibody
      • H2B/j antibody
      • H2B/o antibody
      • H2B/q antibody
      • H2BFB antibody
      • H2BFC antibody
      • H2BFD antibody
      • H2BFE antibody
      • H2BFF antibody
      • H2BFJ antibody
      • H2BFO antibody
      • H2BFQ antibody
      • H2BFS antibody
      • H2BGL105 antibody
      • H2BQ antibody
      • HIRIP2 antibody
      • HIST1H2BB antibody
      • HIST1H2BD antibody
      • HIST1H2BH antibody
      • HIST1H2BL antibody
      • HIST1H2BM antibody
      • HIST1H2BN antibody
      • HIST2H2BE antibody
      • histone cluster 2 antibody
      • Histone H2B antibody
      • histone H2B GL105 antibody
      • Histone H2B type 1 B antibody
      • Histone H2B type 1 D antibody
      • Histone H2B type 1 H antibody
      • Histone H2B type 1 L antibody
      • Histone H2B type 1 M antibody
      • Histone H2B type 1 N antibody
      • Histone H2B type 2 E antibody
      • Histone H2B.q antibody
      • histone protein antibody
      see all

    Images

    • Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
      Western blot - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
      All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335) at 1/2000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with TSA)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 14 kDa
      Observed band size: 17 kDa why is the actual band size different from the predicted?



      This data was developed using ab62335, the same antibody clone in a different buffer formulation.

      The bottom image shows beta Tubulin as a positive control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
      This data was developed using ab62335, the same antibody clone in a different buffer formulation.Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
    • Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)
      Anti-Histone H2B (acetyl K15) antibody [EP955Y] - BSA and Azide free (ab247351)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab247351? Please let us know so that we can cite the reference in this datasheet.

    ab247351 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab247351.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.