Recombinant Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM260] to Histone H2B (acetyl K23)
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human
Overview
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Product name
Anti-Histone H2B (acetyl K23) antibody [RM260]
See all Histone H2B primary antibodies -
Description
Rabbit monoclonal [RM260] to Histone H2B (acetyl K23) -
Host species
Rabbit -
Specificity
ab222770 reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones. -
Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Histone H2B (acetyl K23).
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Positive control
- WB: HeLa cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab222770 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM260 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.5 - 2 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 0.5 - 2 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
- SwissProt: P33778 Human
- SwissProt: P57053 Human
see all -
Alternative names
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
Images
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K23) (red) using ab222770 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).
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All lanes : Anti-Histone H2B (acetyl K23) antibody [RM260] (ab222770) at 0.5 µg/ml
Lane 1 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate
Lane 2 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated
Lane 3 : Recombinant Histone H2B
Predicted band size: 14 kDa -
ab222770 specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-mordified Lysine 23 in Histone H2B.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab222770 has not yet been referenced specifically in any publications.