Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade
See all Histone H2B primary antibodies -
DescriptionRabbit monoclonal [EP857Y] to Histone H2B (acetyl K5) - ChIP Grade
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Host speciesRabbit
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SpecificityThere is cross-reactivity with H3K27Ac (Histone H3 acetylated on Lys 27).
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Tested applicationsSuitable for: WB, IHC - Wholemount, CHIPseq, ChIP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Mouse, Rat, Human, Caenorhabditis elegans
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Immunogen
Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K5). The exact sequence is proprietary.
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Positive control
- TSA-treated A431 cell lysate, human urinary bladder carcinoma.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP857Y
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IsotypeIgG
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Research areas
Associated products
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ChIP Related Products
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Corresponding Antibody
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab40886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). | |
| IHC - Wholemount | Use at an assay dependent concentration. | |
| CHIPseq | Use at an assay dependent concentration. | |
| ChIP | Use 6-25 µg for µg of chromatin. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/250 - 1/500. |
Target
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FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Sequence similaritiesBelongs to the histone H2B family.
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Post-translational
modificationsMonoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. -
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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Database links
- Entrez Gene: 440689 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 104021 Mouse
- Entrez Gene: 64647 Rat
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
- SwissProt: P33778 Human
see all -
Alternative names
- H2B 1A antibody
- H2B antibody
- H2B histone family antibody
see all
Images
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![ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)](https://www.abcam.com/ps/products/40/ab40886/Images/ab40886_1.jpg)
ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 6µl of ab40886 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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![Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)](https://www.abcam.com/ps/products/40/ab40886/Images/ab40886a.gif)
Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/50000 dilution
Lane 1 : A431 cell lysate - untreated
Lane 2 : A431 cell lysate - treated with TSA
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)](https://www.abcam.com/ps/products/40/ab40886/Images/ab40886b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)ab40886 staining human bladder carcinoma for Histone H2B expression (1:250 dilution) -
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)](https://www.abcam.com/ps/products/40/ab40886/Images/ab40886c.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)ab40886 (1:250) staining A431 cells by immunofluorescence. -
![ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)](https://www.abcam.com/ps/products/40/ab40886/Images/Histone-H2B-Primary-antibodies-ab40886-2.jpg)
ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)This image is courtesy of an anonymous abreview.ab40886 at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4°C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.
Detection step: Semiquantitative PCR.Negative control: Rabbit IgG.
Cells treated with active vitamin D3. -
![IHC - Wholemount - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)](https://www.abcam.com/ps/products/40/ab40886/Images/ab40886-214928-ab40886ihcwholemount.jpg)
IHC - Wholemount - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)This image is courtesy of an anonymous AbreviewIHC - Wholemount of Caenorhabditis elegans larvae labelling Histone H2B (acetyl K5) with ab40886. The sample was incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/1000), was used as the secondary antibody.
References
This product has been referenced in:
- Gao J et al. HDAC3 But not HDAC2 Mediates Visual Experience-Dependent Radial Glia Proliferation in the Developing Xenopus Tectum. Front Cell Neurosci 10:221 (2016). IHC-Fr ; Xenopus laevis . Read more (PubMed: 27729849) »
- Shearstone JR et al. Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2. PLoS One 11:e0153767 (2016). ChIP . Read more (PubMed: 27073918) »
Customer reviews and Q&As
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