For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    histone-h2b-acetyl-k5-antibody-ep857y-chip-grade-ab40886.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Histones H2B Acetylated
Share by email
RecombinantRabMAb

Recombinant Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (9)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
  • Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP857Y] to Histone H2B (acetyl K5) - ChIP Grade
  • Suitable for: WB, ChIP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Primary
Product image
Anti-Histone H4 (acetyl K16) antibody [EPR1004] (ab109463)
ChIP
ChIP Kit - Plants (ab117137)
Biochemical
C 646, p300/CBP Inhibitor, p300/CBP HAT inhibitor (ab142163)

View more associated products

Overview

  • Product name

    Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade
    See all Histone H2B primary antibodies
  • Description

    Rabbit monoclonal [EP857Y] to Histone H2B (acetyl K5) - ChIP Grade
  • Host species

    Rabbit
  • Specificity

    There is cross-reactivity with H3K27Ac (Histone H3 acetylated on Lys 27).

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, ChIP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Caenorhabditis elegans, Drosophila melanogaster
  • Immunogen

    Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K5). The exact sequence is proprietary.
    (Peptide available as ab203469)

  • Positive control

    • WB: HeLa (500ng/ml trichostatin A for 4 hours), NIH/3T3 (500ng/ml trichostatin A for 4 hours) and C6 (500ng/ml trichostatin A for 4 hours) cell lysates. IHC-P: Human hepatocellular carcinoma and urinary bladder carcinoma. ICC/IF: HeLa and A431 cells. ChIP: HeLa cells.
  • General notes

    We do not guarantee IHC-P for mouse and rat.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP857Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H2B
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • Alternative Versions

    • Anti-Histone H2B (acetyl K5) antibody [EP857Y] - BSA and Azide free (ab247300)
  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Histone H2B protein (ab92409)

Applications

Our Abpromise guarantee covers the use of ab40886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

For unpurified use at 1/50000.

ChIP Use 2 µg for 25 µg of chromatin.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

ICC/IF 1/1000.

For unpurified use at 1/250 - 1/500.

Target

  • Relevance

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 337874 Human
    • Entrez Gene: 54145 Human
    • Entrez Gene: 8349 Human
    • Entrez Gene: 104021 Mouse
    • Entrez Gene: 64647 Rat
    • SwissProt: P04255 Caenorhabditis elegans
    • SwissProt: P02283 Drosophila melanogaster
    • SwissProt: O60814 Human
    • SwissProt: P06899 Human
    • SwissProt: P23527 Human
    • SwissProt: P33778 Human
    • SwissProt: P57053 Human
    • SwissProt: P58876 Human
    • SwissProt: P62807 Human
    • SwissProt: Q16778 Human
    • SwissProt: Q5QNW6 Human
    • SwissProt: Q8N257 Human
    • SwissProt: Q93079 Human
    • SwissProt: Q99877 Human
    • SwissProt: Q99879 Human
    • SwissProt: Q99880 Human
    • SwissProt: P10854 Mouse
    • SwissProt: Q64475 Mouse
    • SwissProt: Q00729 Rat
    see all
  • Alternative names

    • GL105 antibody
    • H2B GL105 antibody
    • H2B histone family member O antibody
    • H2B histone family member S antibody
    • H2B.1 antibody
    • H2B.1 B antibody
    • H2B.b antibody
    • H2B.c antibody
    • H2B.d antibody
    • H2B.e antibody
    • H2B.f antibody
    • H2B.j antibody
    • H2B.q antibody
    • H2B/b antibody
    • H2B/c antibody
    • H2B/d antibody
    • H2B/e antibody
    • H2B/f antibody
    • H2B/j antibody
    • H2B/o antibody
    • H2B/q antibody
    • H2BFB antibody
    • H2BFC antibody
    • H2BFD antibody
    • H2BFE antibody
    • H2BFF antibody
    • H2BFJ antibody
    • H2BFO antibody
    • H2BFQ antibody
    • H2BFS antibody
    • H2BGL105 antibody
    • H2BQ antibody
    • HIRIP2 antibody
    • HIST1H2BB antibody
    • HIST1H2BD antibody
    • HIST1H2BH antibody
    • HIST1H2BL antibody
    • HIST1H2BM antibody
    • HIST1H2BN antibody
    • HIST2H2BE antibody
    • histone cluster 2 antibody
    • Histone H2B antibody
    • histone H2B GL105 antibody
    • Histone H2B type 1 B antibody
    • Histone H2B type 1 D antibody
    • Histone H2B type 1 H antibody
    • Histone H2B type 1 L antibody
    • Histone H2B type 1 M antibody
    • Histone H2B type 1 N antibody
    • Histone H2B type 2 E antibody
    • Histone H2B.q antibody
    • histone protein antibody
    see all

Images

  • Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 500ng/ml trichostatin A for 4 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

    Chromatin was prepared from HeLa  (Human cervix adenocarcinoma epithelial cell) cells according to the Abcam X-ChIP protocol*. Cells were fixed with formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 2µg of ab40886 (red), or 2 µg of rabbit normal IgG (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling Histone H2B with purified ab40886 at 1/100 dilution (5.42 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 500 ng/mL TSA for 4 hours cells labeling Histone H2B with purified ab40886 at 1/1000 dilution (0.54 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

    PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/10000 dilution (Purified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 500ng/ml trichostatin A for 4 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/10000 dilution (Purified)

    Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 2 : C6 (Rat glial tumor glial cell) treated with 500ng/ml trichostatin A for 4 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

    Chromatin was prepared from HeLa (Human cervix adenocarcinoma epithelial cell) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 6µl of unpurified ab40886 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.    

  • Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Western blot - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/3000 dilution + Drosophila melanogaster lysate (Fruit fly larvae) at 20 µg

    Secondary
    Anti-rabbit IgG HRP at 1/6000 dilution

    Predicted band size: 14 kDa

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

    ab40886 (unpurified) staining human bladder carcinoma for Histone H2B expression (1/250 dilution)

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

    ab40886 (unpurified) (1/250) staining A431 (Human epidermoid carcinoma cell line) cells by immunofluorescence.

  • ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    ChIP - Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)This image is courtesy of an anonymous abreview.

    ab40886 (unpurified) at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4°C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.Detection step: Semiquantitative PCR.Negative control: Rabbit IgG.Cells treated with active vitamin D3.

    See Abreview

  • Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)
    Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (9)

    Publishing research using ab40886? Please let us know so that we can cite the reference in this datasheet.

    ab40886 has been referenced in 9 publications.

    • Zhang S  et al. Epigenetic regulation of REX1 expression and chromatin binding specificity by HMGNs. Nucleic Acids Res 47:4449-4461 (2019). PubMed: 30838422
    • Yucel N  et al. Glucose Metabolism Drives Histone Acetylation Landscape Transitions that Dictate Muscle Stem Cell Function. Cell Rep 27:3939-3955.e6 (2019). PubMed: 31242425
    • Jia H  et al. Ras-ERK1/2 signaling accelerates the progression of colorectal cancer via mediation of H2BK5ac. Life Sci 230:89-96 (2019). PubMed: 31129138
    • Brocks D  et al. DNMT and HDAC inhibitors induce cryptic transcription start sites encoded in long terminal repeats. Nat Genet 49:1052-1060 (2017). PubMed: 28604729
    • Gao J  et al. HDAC3 But not HDAC2 Mediates Visual Experience-Dependent Radial Glia Proliferation in the Developing Xenopus Tectum. Front Cell Neurosci 10:221 (2016). IHC-Fr ; Xenopus laevis . PubMed: 27729849
    • Shearstone JR  et al. Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2. PLoS One 11:e0153767 (2016). ChIP . PubMed: 27073918
    • Zhou P  et al. Proportions of acetyl-histone-positive hepatocytes indicate the functional status and prognosis of cirrhotic patients. World J Gastroenterol 21:6665-74 (2015). PubMed: 26074705
    • Santoro SW & Dulac C The activity-dependent histone variant H2BE modulates the life span of olfactory neurons. elife 1:e00070 (2012). PubMed: 23240083
    • Wang Z  et al. Combinatorial patterns of histone acetylations and methylations in the human genome. Nat Genet 40:897-903 (2008). ChIP-sequencing . PubMed: 18552846

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Histone H2B (acetyl K5) [EP857Y] antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Caenorhabditis elegans Embryo (C. elegans larvae)
    Specification
    C. elegans larvae
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 30 2013

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Histone H2B (acetyl K5) [EP857Y] antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step
    None
    Sample
    Rat Tissue sections (Rat olfactory bulb frozen section)
    Specification
    Rat olfactory bulb frozen section
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 30 2013

    Immunocytochemistry abreview for Anti-Histone H2B (acetyl K5) [EP857Y] antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample
    Human Cultured Cells (Human embryonic stem cells)
    Specification
    Human embryonic stem cells
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 27 2013

    ChIP abreview for Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Tissue lysate - whole (dorsal skin epidermis)
    Specification
    dorsal skin epidermis
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step
    Semiquantitative PCR
    Negative control
    Rabbit IgG
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.