Product nameAnti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade
See all Histone H2B primary antibodies
DescriptionRabbit monoclonal [EP857Y] to Histone H2B (acetyl K5) - ChIP Grade
SpecificityThere is cross-reactivity with H3K27Ac (Histone H3 acetylated on Lys 27).
Tested applicationsSuitable for: WB, IHC - Wholemount, CHIPseq, ChIP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human, Caenorhabditis elegans
Synthetic peptide within Human Histone H2B aa 1-100 (acetyl K5). The exact sequence is proprietary.
- TSA-treated A431 cell lysate, human urinary bladder carcinoma.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
ChIP Related Products
Our Abpromise guarantee covers the use of ab40886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).|
|IHC - Wholemount||Use at an assay dependent concentration.|
|CHIPseq||Use at an assay dependent concentration.|
|ChIP||Use 6-25 µg for µg of chromatin.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/250 - 1/500.|
RelevanceCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
- H2B GL105 antibody
- H2B histone family member O antibody
- H2B histone family member S antibody
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 6µl of ab40886 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/50000 dilution
Lane 1 : A431 cell lysate - untreated
Lane 2 : A431 cell lysate - treated with TSA
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa
ab40886 staining human bladder carcinoma for Histone H2B expression (1:250 dilution)
ab40886 (1:250) staining A431 cells by immunofluorescence.
ab40886 at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4°C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.
Detection step: Semiquantitative PCR.Negative control: Rabbit IgG.
Cells treated with active vitamin D3.
IHC - Wholemount of Caenorhabditis elegans larvae labelling Histone H2B (acetyl K5) with ab40886. The sample was incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/1000), was used as the secondary antibody.
This product has been referenced in:
- Brocks D et al. DNMT and HDAC inhibitors induce cryptic transcription start sites encoded in long terminal repeats. Nat Genet 49:1052-1060 (2017). Read more (PubMed: 28604729) »
- Gao J et al. HDAC3 But not HDAC2 Mediates Visual Experience-Dependent Radial Glia Proliferation in the Developing Xenopus Tectum. Front Cell Neurosci 10:221 (2016). IHC-Fr ; Xenopus laevis . Read more (PubMed: 27729849) »