Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Histone H2B (acetyl K5) [EP857Y] antibody - ChIP Grade

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat olfactory bulb frozen section)
Specification
Rat olfactory bulb frozen section
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA and 0.1% Triton 100 in TBS buffer
Dilution
1/200

Secondary antibody

Dilution
1/1000

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Submitted Dec 30 2013

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