Western blot abreview for Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly larvae)
Specification
Fruit fly larvae
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% BSA
Dilution
1/3000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/6000

Detection

Detection method
ECL
Bands
Specific: 15 kDa
Exposure
1 minute(s) and 0 second(s)

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Submitted Jan 08 2015

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