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    histone-h2b-antibody-mabcam-52484-chip-grade-ab52484.pdf

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Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)

  • Datasheet
  • SDS
Reviews (11) Submit a question References (17)

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  • ChIP - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
  • Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
  • Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
  • Flow Cytometry - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
  • Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
  • Immunoprecipitation - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)

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  • Datasheet
  • References (17)
  • Protocols

Overview

  • Product name

    Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade
    See all Histone H2B primary antibodies
  • Description

    Mouse monoclonal [mAbcam 52484] to Histone H2B - ChIP Grade
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, Flow Cyt, WB, ICC/IF, ChIP, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human, Drosophila melanogaster, Zebrafish
    Predicted to work with: Cow, Xenopus laevis, Caenorhabditis elegans, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Histone H2B aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • This antibody gave a positive signal in NIH3T3 and PC12 whole cell lysates and when tested against Histone H2B recombinant protein.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 52484
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • Alternative Versions

    • Anti-Histone H2B antibody [mAbcam 52484] (Alexa Fluor® 647) (ab202567)
    • Anti-Histone H2B antibody [mAbcam 52484] (Alexa Fluor® 488) (ab204258)
    • Anti-Histone H2B antibody [mAbcam 52484] (HRP) (ab204463)
    • Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade – BSA and Azide free (ab237964)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Immunohistochemistry kits

    • Mouse on Mouse Polymer IHC Kit (ab127055)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Histone H2B protein (ab92409)

Applications

Our Abpromise guarantee covers the use of ab52484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
ICC/IF Use a concentration of 1 µg/ml.
ChIP Use 5 µg for µg of chromatin.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 337874 Human
    • Entrez Gene: 54145 Human
    • Entrez Gene: 8349 Human
    • Entrez Gene: 104021 Mouse
    • Entrez Gene: 64647 Rat
    • SwissProt: P04255 Caenorhabditis elegans
    • SwissProt: P62808 Cow
    • SwissProt: P02283 Drosophila melanogaster
    • SwissProt: O60814 Human
    • SwissProt: P06899 Human
    • SwissProt: P23527 Human
    • SwissProt: P33778 Human
    • SwissProt: P57053 Human
    • SwissProt: P58876 Human
    • SwissProt: P62807 Human
    • SwissProt: Q16778 Human
    • SwissProt: Q5QNW6 Human
    • SwissProt: Q8N257 Human
    • SwissProt: Q93079 Human
    • SwissProt: Q99877 Human
    • SwissProt: Q99879 Human
    • SwissProt: Q99880 Human
    • SwissProt: P10854 Mouse
    • SwissProt: Q64475 Mouse
    • SwissProt: Q00729 Rat
    see all
  • Alternative names

    • GL105 antibody
    • H2B GL105 antibody
    • H2B histone family member O antibody
    • H2B histone family member S antibody
    • H2B.1 antibody
    • H2B.1 B antibody
    • H2B.b antibody
    • H2B.c antibody
    • H2B.d antibody
    • H2B.e antibody
    • H2B.f antibody
    • H2B.j antibody
    • H2B.q antibody
    • H2B/b antibody
    • H2B/c antibody
    • H2B/d antibody
    • H2B/e antibody
    • H2B/f antibody
    • H2B/j antibody
    • H2B/o antibody
    • H2B/q antibody
    • H2BFB antibody
    • H2BFC antibody
    • H2BFD antibody
    • H2BFE antibody
    • H2BFF antibody
    • H2BFJ antibody
    • H2BFO antibody
    • H2BFQ antibody
    • H2BFS antibody
    • H2BGL105 antibody
    • H2BQ antibody
    • HIRIP2 antibody
    • HIST1H2BB antibody
    • HIST1H2BD antibody
    • HIST1H2BH antibody
    • HIST1H2BL antibody
    • HIST1H2BM antibody
    • HIST1H2BN antibody
    • HIST2H2BE antibody
    • histone cluster 2 antibody
    • Histone H2B antibody
    • histone H2B GL105 antibody
    • Histone H2B type 1 B antibody
    • Histone H2B type 1 D antibody
    • Histone H2B type 1 H antibody
    • Histone H2B type 1 L antibody
    • Histone H2B type 1 M antibody
    • Histone H2B type 1 N antibody
    • Histone H2B type 2 E antibody
    • Histone H2B.q antibody
    • histone protein antibody
    see all

Images

  • ChIP - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    ChIP - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 5µg of  ab52484 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.  

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)This image is courtesy of an anonymous Abreview

    ab52484 staining Histone H2B in Fruit fly (Drosophila melanogaster) embryo cells by ICC/IF (Immunocytochemistry/immunofluorescence). Embryos were washed in 1x PBS + 0.1% Trition, cells were fixed with heat, and blocked with 10% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/1000) for 24 hours. An Alexa Fluor®594-conjugated Rabbit anti-mouse IgG polyclonal (1/5000) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    All lanes : Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484) at 5 µg/ml

    Lane 1 : Histone H1 recombinant protein.
    Lane 2 : Histone H2A recombinant protein.
    Lane 3 : Histone H2B recombinant protein.
    Lane 4 : Histone H3 recombinant protein.
    Lane 5 : Histone H4 recombinant protein.

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)

    ICC/IF image of ab52484 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab52484, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    IHC image of Histone H2B staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52484, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    All lanes : Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484) at 5 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 10 µg
    Lane 4 : Zebrafish liver homogenate at 10 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 10 µg
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Flow Cytometry - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Flow Cytometry - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Overlay histogram showing HeLa cells stained with ab52484 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52484, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Western blot - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    All lanes : Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Immunoprecipitation - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Immunoprecipitation - Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)
    Histone H2B was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Histone H2B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52484.
    Secondary: Protein G-HRP at 1/500 dilution.
    Band: 17kDa; Histone H2B; non specific bands - 26kDa: We are unsure as to the identity of this extra band.

Protocols

  • Mouse on Mouse staining protocol
  • ChIP protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References

    This product has been referenced in:

    • Pefani DE  et al. MST2 kinase suppresses rDNA transcription in response to DNA damage by phosphorylating nucleolar histone H2B. EMBO J 37:N/A (2018). Read more (PubMed: 29789391) »
    • Travers J  et al. Chromatin regulates IL-33 release and extracellular cytokine activity. Nat Commun 9:3244 (2018). Read more (PubMed: 30108214) »
    See all 17 Publications for this product

    Publishing research using ab52484? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 11 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Schmidtea mediterranea Cell (whole mount)
    Permeabilization
    No
    Specification
    whole mount
    Blocking step
    BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Carnoy's
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 14 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (lung tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate
    Permeabilization
    No
    Specification
    lung tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Jim Hsiao

    Verified customer

    Submitted Dec 13 2017

    ChIP abreview for Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - whole cell (Mouse colon cells)
    Specification
    Mouse colon cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde 11%
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 30 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Histone H2B [mAbcam 52484] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Colon)
    Specification
    Colon
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 06 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-Histone H2B [mAbcam 52484] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Breast)
    Specification
    Breast
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 20%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 26 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Sample
    Fruit fly (Drosophila melanogaster) Cell (Drosophila Embryo)
    Specification
    Drosophila Embryo
    Permeabilization
    No
    Fixative
    Heat
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 09 2013

    Western blot abreview for Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse lung)
    Loading amount
    100000 cells
    Specification
    Mouse lung
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 22 2012

    ChIP abreview for Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (Breast cell line)
    Specification
    Breast cell line
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Detection step
    Real-time PCR
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 20 2012

    Western blot abreview for Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cell line)
    Loading amount
    100000 cells
    Specification
    Breast cell line
    Gel Running Conditions
    Non-reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 20 2012

    Western blot abreview for Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chicken Cell lysate - nuclear (QT6 cells)
    Loading amount
    7.5 µg
    Specification
    QT6 cells
    Gel Running Conditions
    Reduced Denaturing (12% acrylamide)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 14 2012

    1-10 of 11 Abreviews or Q&A

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