Overview

Description

  • Nature

    Synthetic
  • Amino Acid Sequence

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab203370 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-Histone H2B (crotonyl K5) antibody [EPR17483] (ab177396)

    • Form

      Liquid
    • Additional notes

      This is the blocking peptide for ab177396

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C.

    General Info

    • Alternative names

      • GL105
      • H2B GL105
      • H2B histone family member O
      • H2B histone family member S
      • H2B.1
      • H2B.1 B
      • H2B.b
      • H2B.c
      • H2B.d
      • H2B.e
      • H2B.f
      • H2B.j
      • H2B.q
      • H2B/b
      • H2B/c
      • H2B/d
      • H2B/e
      • H2B/f
      • H2B/j
      • H2B/o
      • H2B/q
      • H2BFB
      • H2BFC
      • H2BFD
      • H2BFE
      • H2BFF
      • H2BFJ
      • H2BFO
      • H2BFQ
      • H2BFS
      • H2BGL105
      • H2BQ
      • HIRIP2
      • HIST1H2BB
      • HIST1H2BD
      • HIST1H2BH
      • HIST1H2BL
      • HIST1H2BM
      • HIST1H2BN
      • HIST2H2BE
      • histone cluster 2
      • Histone H2B
      • histone H2B GL105
      • Histone H2B type 1 B
      • Histone H2B type 1 D
      • Histone H2B type 1 H
      • Histone H2B type 1 L
      • Histone H2B type 1 M
      • Histone H2B type 1 N
      • Histone H2B type 2 E
      • Histone H2B.q
      • histone protein
      see all
    • Relevance

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
    • Cellular localization

      Nuclear

    References

    ab203370 has not yet been referenced specifically in any publications.

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