This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Histone H2B (formyl K34) antibody [EP17607] (ab177437)

Overview

  • Product name
    Anti-Histone H2B (formyl K34) antibody [EP17607]
    See all Histone H2B primary antibodies
  • Description
    Rabbit monoclonal [EP17607] to Histone H2B (formyl K34)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species
    Rabbit
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Histone H2B aa 1-100 (formyl K34) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16778

  • Positive control
    • WB: Mouse embryo, testis and ovary lysates; F9, mESC, C2C12, 3T3-L1, NIH/3T3, HeLa, NT2/D1, HL-60 and NCCIT whole cell lysates; human ovary lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EP17607
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
PepArr Use at an assay dependent concentration.

Target

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-Histone H2B (formyl K34) antibody [EP17607] (ab177437) at 1/1000 dilution

    Lane 1 : Mouse embryo lysate
    Lane 2 : Mouse testis lysate
    Lane 3 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
    Lane 4 : mESC (Mouse embryonic stem cell line) whole cell lysate
    Lane 5 : Mouse ovary lysate
    Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 7 : 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 9 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 10 : Human placenta lysate
    Lane 11 : NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 12 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
    Lane 13 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 14 : Human ovary lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% BSA/TBST.

  • ab177437 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

References

ab177437 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab177437.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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