Product nameAnti-Histone H2B (formyl K34) antibody [EP17607]
See all Histone H2B primary antibodies
DescriptionRabbit monoclonal [EP17607] to Histone H2B (formyl K34)
Tested applicationsSuitable for: WB, PepArrmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Histone H2B aa 1-100 (formyl K34) (Cysteine residue). The exact sequence is proprietary.
Database link: Q16778
- WB: Mouse embryo, testis and ovary lysates; F9, mESC, C2C12, 3T3-L1, NIH/3T3, HeLa, NT2/D1, HL-60 and NCCIT whole cell lysates; human ovary lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab177437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).|
|PepArr||Use at an assay dependent concentration.|
RelevanceCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
- H2B GL105 antibody
- H2B histone family member O antibody
- H2B histone family member S antibody
All lanes : Anti-Histone H2B (formyl K34) antibody [EP17607] (ab177437) at 1/1000 dilution
Lane 1 : Mouse embryo lysate
Lane 2 : Mouse testis lysate
Lane 3 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lane 4 : mESC (Mouse embryonic stem cell line) whole cell lysate
Lane 5 : Mouse ovary lysate
Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 7 : 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 9 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 10 : Human placenta lysate
Lane 11 : NT2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 12 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lane 13 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 14 : Human ovary lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% BSA/TBST.
ab177437 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
ab177437 has not yet been referenced specifically in any publications.