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Rabbit polyclonal to Histone H2B (formyl K5)
Mouse, Cow, Human
Predicted to work with:
Synthetic peptide within Human Histone H2B aa 1 to the C-terminus (formyl K5) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q16778
This antibody gave a positive signal in HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) and Calf Thymus histone (nuclear) lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
H2B type 12 antibody
Western blot - Anti-Histone H2B (formyl K5) antibody (ab177138)
All lanes :
Anti-Histone H2B (formyl K5) antibody (ab177138) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 :
HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg
Lane 3 :
NIH 3T3 (Mouse) Whole Cell Lysate at 10 µg
Lane 4 :
Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (
) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
18 kDa (
why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177138 overnight at 4°C. Antibody binding was detected using
ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
has not yet been referenced specifically in any publications.
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