Anti-Histone H2B (formyl K5) antibody (ab177138)

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Overview

  • Product name
    Anti-Histone H2B (formyl K5) antibody
    See all Histone H2B primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (formyl K5)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Histone H2B aa 1 to the C-terminus (formyl K5) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q16778

  • Positive control
    • This antibody gave a positive signal in HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) and Calf Thymus histone (nuclear) lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).

Target

Images

  • Western blot - Anti-Histone H2B (formyl K5) antibody (ab177138)
    Western blot - Anti-Histone H2B (formyl K5) antibody (ab177138)
    All lanes : Anti-Histone H2B (formyl K5) antibody (ab177138) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) at 10 µg
    Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate at 10 µg
    Lane 4 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 18 kDa (why is the actual band size different from the predicted?)



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177138 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

References

ab177138 has not yet been referenced specifically in any publications.

Publishing research using ab177138? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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