Recombinant Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)
![Peptide Array - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)](https://www.abcam.com/ps/products/202/ab202118/Images/ab202118-270922-anti-histone-h2b-mono-methyl-k23-antibody-epr17603-peptide-array.jpg "Peptide Array - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17603] to Histone H2B (mono methyl K23)
- Suitable for: WB, ICC/IF, PepArr
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Histone H2B (mono methyl K23) antibody [EPR17603]
See all Histone H2B primary antibodies -
Description
Rabbit monoclonal [EPR17603] to Histone H2B (mono methyl K23) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, PepArrmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Histone H2B aa 1-100 (mono methyl K23). The exact sequence is proprietary.
Database link: Q16778 -
Positive control
- WB: HeLa and NIH/3T3 whole cell lysates. ICC/IF: HeLa and NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17603 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab202118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). | |
| ICC/IF | 1/4000. | |
| PepArr | Use a concentration of 0.2 µg/ml. |
Target
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Relevance
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- Entrez Gene: 104021 Mouse
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
- SwissProt: P33778 Human
see all -
Alternative names
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
Images
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Peptide Array - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)
ab202118 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)](https://www.abcam.com/ps/products/202/ab202118/Images/ab202118-270578-anti-histone-h2b-mono-methyl-k23-antibody-epr17603-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H2B (mono methyl K23) with ab202118 at 1/4000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)](https://www.abcam.com/ps/products/202/ab202118/Images/ab202118-270577-anti-histone-h2b-mono-methyl-k23-antibody-epr17603-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Histone H2B (mono methyl K23) with ab202118 at 1/4000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Western blot - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)](https://www.abcam.com/ps/products/202/ab202118/Images/ab202118-270580-anti-histone-h2b-mono-methyl-k23-antibody-epr17603-western-blot.jpg)
Western blot - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% BSA/TBST.
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![Western blot - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)](https://www.abcam.com/ps/products/202/ab202118/Images/ab202118-270579-anti-histone-h2b-mono-methyl-k23-antibody-epr17603-western-blot.jpg)
Western blot - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118) at 1/1000 dilution + NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% BSA/TBST.
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![Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)](https://www.abcam.com/ps/products/202/ab202118/Images/ab202118-7-benefits-of-recombinant-antibodies.png)
Anti-Histone H2B (mono methyl K23) antibody [EPR17603] (ab202118)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab202118 has not yet been referenced specifically in any publications.
