Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM238] to Histone H2B (phospho S14)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-Histone H2B (phospho S14) antibody [RM238]
See all Histone H2B primary antibodies
DescriptionRabbit monoclonal [RM238] to Histone H2B (phospho S14)
Specificityab222762 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Histone H2B (phospho S14).
- WB: Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Purification notesab222762 was purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab222762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
RelevanceCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2B (phospho S14) (red) using ab222762 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green). DAPI nuclear stain (blue).
All lanes : Anti-Histone H2B (phospho S14) antibody [RM238] (ab222762) at 0.5 µg/ml
Lane 1 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole
Lane 2 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole
Predicted band size: 14 kDa
ab222762 has not yet been referenced specifically in any publications.