• Product name
    Anti-Histone H2B (testis specific) antibody
    See all Histone H2B (testis specific) primary antibodies
  • Description
    Rabbit polyclonal to Histone H2B (testis specific)
  • Host species
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H2B (testis specific) aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab24027, ab24027)

  • Positive control
    • Testis Tissue Lysate (Mouse)



Our Abpromise guarantee covers the use of ab23913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/3000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).


  • Function
    Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells. Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones. Also expressed maternally and is present in the female pronucleus, suggesting a similar role in protamine replacement by nucleosomes at fertilization (By similarity). Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Tissue specificity
    Mainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level). Also found in some fat cells.
  • Sequence similarities
    Belongs to the histone H2B family.
  • Post-translational
    Monoubiquitination at Lys-36 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-122 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
    Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
    Acetylated during spermatogenesis. Acetylated form is most abundant in spermatogonia compared to spermatocytes and round spermatids.
    Phosphorylated at Thr-117 in spermatogonia, spermatocytes and round spermatids.
    Methylated at Lys-118 in spermatogonia, spermatocytes and round spermatids.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIST1H2BA antibody
    • testis antibody
    • bA317E16.3 antibody
    • H2B antibody
    • H2B histone family member U antibody
    • H2B histone family member U testis specific antibody
    • H2B histone family, member U, (testis specific) antibody
    • H2B testis antibody
    • H2B1A_HUMAN antibody
    • H2BFU antibody
    • H2BT antibody
    • HIST1H2BA antibody
    • Histone 1, H2ba antibody
    • Histone cluster 1 H2ba antibody
    • Histone H2B antibody
    • Histone H2B testis antibody
    • Histone H2B type 1 A antibody
    • Histone H2B type 1-A antibody
    • Histone H2B type 1A antibody
    • STBP antibody
    • Testis specific histone H2B antibody
    • Testis-specific histone H2B antibody
    • TSH 2B antibody
    • TSH2B antibody
    • TSH2B.1 antibody
    see all


  • All lanes : Anti-Histone H2B (testis specific) antibody (ab23913) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg
    Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg/ml with Human Histone H2B (testis specific) peptide (ab24027) at 1 µg/ml
    Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg with Human Histone H2B peptide (ab13336) at 1 µg/ml
    Lane 4 : HeLa Whole Cell Lysate at 20 µg

    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 17 kDa

    ab23913 specifically recognises mouse Histone H2B (lane1). The signal is efficiently blocked using the immunizing peptide (lane2) but not the corresponding general human H2B peptide (lane3). This indicates that ab23913 specifically recognises testis specific H2B. To support this ab23913 does not recognise human Histone H2B in HeLa whole cell lysates (lane4).
  • Image courtesy of Human Protein Atlas

    ab23913 staining Histone H2B (testis specific) in human testis tissue, showing a distinct and strong staining pattern within the seminiferous tubules. Paraffin embedded human testis was incubated with ab3167 (1/3000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab23913 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org








This product has been referenced in:
  • Kutchy NA  et al. Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study. Reprod Biol Endocrinol 15:59 (2017). Read more (PubMed: 28764714) »
  • Iuso D  et al. Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures. Cell Rep 13:1765-71 (2015). Read more (PubMed: 26628361) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting Abcam.

I've compiled a list of primary antibodies that have been tested in mouse tissue via western blot. The only ovary-specific antibodies that I found were anti-human (with only 50% homology with the respective mouse protein), so the ovary antibody listed below is not exclusively-specific to ovary but also gives a positive signal in kidney, placenta, and small intestine:

1. Liver: anti-albumin ab135575 (https://www.abcam.com/albumin-antibody-c-terminal-ab135575.html)
2. Brain: anti-neuron specific beta III tubulin ab18207 (https://www.abcam.com/neuron-specific-beta-iii-tubulin-antibody-neuronal-marker-ab18207.html)
3. Kidney: anti-SLC14A2 ab95365 (https://www.abcam.com/slc14a2-antibody-ab95365.html)
4. Testis: anti-histone H2B ab23913 (https://www.abcam.com/histone-h2b-testis-specific-antibody-ab23913.html)
5. Ovary: anti-ENPP2 (also kidney, placenta, small intestine): ab77104 (https://www.abcam.com/enpp2-antibody-5h3-ab77104.html)

The advantage of this combination of antibodies is that you can use the same anti-rabbit secondary since they were all raised in a rabbit host. Our anti-rabbit secondary antibodies are listed in the link below:

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link:

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Thank you for your enquiry. Currently we only have a H2B testis specific antibody. It has been tested and is guaranteed to work in mouse cells. We have antibodies against histones and histone modifications. However, this is the only testis specific antibody. I have performed an alignment and I can tell you that there is only a single residue change in the region where the immunogen was derived. Please see the link below; http://www.ebi.ac.uk/cgi-bin/jobresults/clustalw/clustalw-20070216-11383201.aln I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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