Product nameAnti-Histone H2B (testis specific) antibody
See all Histone H2B (testis specific) primary antibodies
DescriptionRabbit polyclonal to Histone H2B (testis specific)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
- Testis Tissue Lysate (Mouse)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab23913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/3000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
FunctionVariant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells. Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones. Also expressed maternally and is present in the female pronucleus, suggesting a similar role in protamine replacement by nucleosomes at fertilization (By similarity). Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Tissue specificityMainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level). Also found in some fat cells.
Sequence similaritiesBelongs to the histone H2B family.
modificationsMonoubiquitination at Lys-36 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-122 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Acetylated during spermatogenesis. Acetylated form is most abundant in spermatogonia compared to spermatocytes and round spermatids.
Phosphorylated at Thr-117 in spermatogonia, spermatocytes and round spermatids.
Methylated at Lys-118 in spermatogonia, spermatocytes and round spermatids.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- HIST1H2BA antibody
- testis antibody
- bA317E16.3 antibody
All lanes : Anti-Histone H2B (testis specific) antibody (ab23913) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg/ml with Human Histone H2B (testis specific) peptide (ab24027) at 1 µg/ml
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg with Human Histone H2B peptide (ab13336) at 1 µg/ml
Lane 4 : HeLa Whole Cell Lysate at 20 µg
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
ab23913 specifically recognises mouse Histone H2B (lane1). The signal is efficiently blocked using the immunizing peptide (lane2) but not the corresponding general human H2B peptide (lane3). This indicates that ab23913 specifically recognises testis specific H2B. To support this ab23913 does not recognise human Histone H2B in HeLa whole cell lysates (lane4).
Image courtesy of Human Protein Atlas
ab23913 staining Histone H2B (testis specific) in human testis tissue, showing a distinct and strong staining pattern within the seminiferous tubules. Paraffin embedded human testis was incubated with ab3167 (1/3000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab23913 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
This product has been referenced in:
- Kutchy NA et al. Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study. Reprod Biol Endocrinol 15:59 (2017). Read more (PubMed: 28764714) »
- Iuso D et al. Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures. Cell Rep 13:1765-71 (2015). Read more (PubMed: 26628361) »