Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18094] to Histone H2B (yeast) - ChIP Grade
- Suitable for: PepArr, ChIP, WB
- Reacts with: Saccharomyces cerevisiae
Product nameAnti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade
See all Histone H2B (yeast) primary antibodies
DescriptionRabbit monoclonal [EPR18094] to Histone H2B (yeast) - ChIP Grade
Tested applicationsSuitable for: PepArr, ChIP, WBmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Saccharomyces cerevisiae Histone H2B (yeast) aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P02293
- WB: Saccharomyces cerevisiae whole cell lysate. ChIP: Chromatin prepared from Saccharomyces cerevisiae cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab188291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|PepArr||Use at an assay dependent concentration.|
|ChIP||Use 2 µg for 25 µg of chromatin.|
|WB||1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H2B family.
modificationsMonoubiquitinated by the RAD6/UBC2-BRE1 complex to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and H3K79me formation. H2BK123ub1 also modulates the formation of double-strand breaks during meiosis and is a prerequisite for DNA-damage checkpoint activation. Deubiquitination is performed by UBP8 in presence of SGF11.
Phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase. May be correlated with chromosome condensation. H2BS10ph is also formed after H(2)O(2) treatment, and is a step leading to apoptosis.
Acetylated by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. H2BK16ac can also be formed by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
Sumoylation to form H2BK6su or H2BK7su, and probably also H2BK16su or H2BK17su, occurs preferentially near the telomeres and represses gene transcription.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H2B1_YEAST antibody
- Histone H2B.1 antibody
- Histone H2B.2 antibody
Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab188291 (red), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
ab188291 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291) at 1/2000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
ab188291 has been referenced in 2 publications.
- Jing Y et al. Site-Specific Installation of Succinyl Lysine Analog into Histones Reveals the Effect of H2BK34 Succinylation on Nucleosome Dynamics. Cell Chem Biol 25:166-174.e7 (2018). PubMed: 29249693
- Shen D & Skibbens RV Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition. PLoS One 12:e0188739 (2017). ChIP . PubMed: 29186203