Recombinant
RabMAb

Recombinant Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291)

Overview

  • Product name

    Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade
    See all Histone H2B (yeast) primary antibodies
  • Description

    Rabbit monoclonal [EPR18094] to Histone H2B (yeast) - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: PepArr, ChIP, WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Saccharomyces cerevisiae Histone H2B (yeast) aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P02293

  • Positive control

    • WB: Saccharomyces cerevisiae whole cell lysate. ChIP: Chromatin prepared from Saccharomyces cerevisiae cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
PepArr Use at an assay dependent concentration.
ChIP Use 2 µg for 25 µg of chromatin.
WB 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H2B family.
  • Post-translational
    modifications

    Monoubiquitinated by the RAD6/UBC2-BRE1 complex to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and H3K79me formation. H2BK123ub1 also modulates the formation of double-strand breaks during meiosis and is a prerequisite for DNA-damage checkpoint activation. Deubiquitination is performed by UBP8 in presence of SGF11.
    Phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase. May be correlated with chromosome condensation. H2BS10ph is also formed after H(2)O(2) treatment, and is a step leading to apoptosis.
    Acetylated by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. H2BK16ac can also be formed by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
    Sumoylation to form H2BK6su or H2BK7su, and probably also H2BK16su or H2BK17su, occurs preferentially near the telomeres and represses gene transcription.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

    • Alternative names

      • H2B1_YEAST antibody
      • Histone H2B.1 antibody
      • Histone H2B.2 antibody
      • HTB 1 antibody
      • HTB 2 antibody
      • HTB1 antibody
      • Htb1p antibody
      • HTB2 antibody
      • Htb2p antibody
      • SPT 12 antibody
      • SPT12 antibody
      • Suppressor of Ty protein 12 antibody
      see all

    Images

    • Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab188291 (red), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    • ab188291 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
       
      Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
       
      The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

    • Anti-Histone H2B (yeast) antibody [EPR18094] - ChIP Grade (ab188291) at 1/2000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:

    • Shen D & Skibbens RV Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition. PLoS One 12:e0188739 (2017). ChIP . Read more (PubMed: 29186203) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab188291.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up