Synthetic peptide within Human Histone H3 (acetyl K14). The exact sequence is proprietary.
(Peptide available as
A trial size is available to purchase for this antibody.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab52946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 19188451|
|WB||1/2000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/250 - 1/500.|
Immunocytochemistry/Immunofluorescence analysis of untreated HeLa cells and TSA (500ng/ml, 4h) and treated HeLa cells labelling Histone H3 (acetyl K14) with purified ab52946 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, counterstained with ab150120 AlexaFluor®594 Goat anti-Mouse secondary 1:1000 (2ug/ml). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Negative Control 1: Rabbit primary antibody and anti-mouse secondary antibody(ab150120)
ab52946 immunoprecipitating Histone H3 (acetyl K14) in Drosophila melanogaster embryo whole tissue lysate. 500µg of tissue lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 2 hours at 4°C. For western blotting ab52946 (1/20000) was used to confirm successful immunoprecipation.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"