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what I should use for a positive control? We use this antibodies for parafine section of breast cancers ab1191 - h3 (acetyl K18) ab1761 - h4 (acetyl K12) ab4441 - h3 (acetyl K9) ab7766 - h3 (dimethyl K4)
Asked on Sep 20 2005
These histone modifications are ubiquitous across all cell types (except yeast which do not have acetylation at lysine 12 of H4). You could use a standard HeLa cell line and prepare them as detailed in the abcam immunocytochemistry protocol (http://ops.abcam.com/index.html?pageconfig=view_protocol&pid=159) or alternatively, http://ops.abcam.com/index.html?pageconfig=protocols&pid=174&intAbID=7766&strTab=protocols&mode=prot It is worth noting that you can elevate the level of histone acetylation across all of the lysine residues you have antibodies against by incubating (if possible) with Na Butyrate (5mM). This will in turn improve your levels of staining.
Answered on Sep 21 2005