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Epigenetics and Nuclear Signaling Histones H3 Acetylated
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Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

  • Datasheet
  • SDS
Reviews (67)Q&A (8)References (1170)

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ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Peptide Array - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

Key features and details

  • Rabbit polyclonal to Histone H3 (acetyl K27) - ChIP Grade
  • Suitable for: ICC/IF, WB, IHC-P, ChIP, PepArr
  • Reacts with: Mouse, Cow, Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H3 (acetyl K27) antibody - ChIP Grade
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (acetyl K27) - ChIP Grade
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    PepArr
    Recombinant fragment
    WB
    Cow
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 aa 1-100 (acetyl K27) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24404)

  • General notes

    Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Signal Transduction
    • Antibodies
    • histone h3

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Control Peptide

    • Human Histone H4 (acetyl K12) peptide (ab15662)
    • Human Histone H3 (acetyl K9) peptide (ab16635)
    • Human Histone H3 (acetyl K23) peptide (ab48359)
  • Immunizing Peptide (Blocking)

    • Human Histone H3 (acetyl K27) peptide (ab24404)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • GAPDH positive control ChIP primer pair (ab267832)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Human Histone H3 (tri methyl K4) peptide (ab1342)
    • Human Histone H3 (tri methyl K9) peptide (ab1773)
    • Human Histone H3 (tri methyl K27) peptide (ab1782)
    • Human Histone H3 (unmodified) peptide (ab7228)
    • Human Histone H3 (di methyl K4) peptide (ab7768)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ChIP
Human
ICC/IF
Mouse
Human
IHC-P
Human
PepArr
Recombinant fragment
WB
Cow
Human
All applications
Rat
Chicken
Xenopus laevis
Arabidopsis thaliana
Drosophila melanogaster
Monkey
Zebrafish
Plasmodium falciparum
Rice
Cyanidioschyzon merolae
Application Abreviews Notes
ICC/IF (15)
Use a concentration of 0.5 µg/ml.

Can be used with paraformaldehyde- or methanol- fixed cells.

WB (17)
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

IHC-P (2)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP (26)
Use 2 µg for 25 µg of chromatin.

We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control.

PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
Notes
ICC/IF
Use a concentration of 0.5 µg/ml.

Can be used with paraformaldehyde- or methanol- fixed cells.

WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP
Use 2 µg for 25 µg of chromatin.

We recommend GAPDH positive control ChIP primer pair ab267832 as a positive control.

PepArr
Use a concentration of 0.2 - 0.02 µg/ml.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H3 family.
  • Developmental stage

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • Post-translational
    modifications

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • Alternative names

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

Images

  • ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4729 (blue), and 20 µl of Protein A/G sepharose beads.

    No antibody was added to the beads control (yellow).

    The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    ab4729 staining Histone H3 (acetyl K27) in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were incubated with 10 mM sodium butyrate (ab120948) for 6 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5 minutes) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab4729 at 0.5 µg/ml and ab7291 at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with a anti-rabbit AlexaFluor®488 secondary antibody (ab150077) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor®594 (ab150120) at 2 μg/ml (shown in pseudo colour red). Nuclear DNA was labeled in blue with DAPI.

    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    IHC image of ab4729 staining Histone H3 (acetyl K27) in human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer pH 6 for 20 minutes. The section was then incubated with ab4729, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Peptide Array - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Peptide Array - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    All batches of ab4729 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - acetyl K27 peptide (ab24404), indicating that this antibody specifically recognises the Histone H3 - acetyl K27 modification.

    ab24404 - Histone H3 - acetyl K27

    ab15591 - Histone H3 - acetyl K14

    ab24003 - Histone H3 - acetyl K18

    ab17163 - Histone H3 unmodified

    ab48359 - Histone H3 - acetyl K23

    ab41409 - Histone H3 - acetyl K36

    ab15662 - Histone H4 - acetyl K12

    ab16635 - Histone H3 acetyl K9

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    All lanes : Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 1/2500 dilution

    Lane 1 : Untreated Mouse MEF cell lysate
    Lane 2 : 0.4 µM Trichostatin A treatment for 18 hr Mouse MEF cell lysate

    Lysates/proteins at 9 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution

    Predicted band size: 15 kDa

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)This image is courtesy of Petra Hajkova - Gurdon Institute, Cambridge University

    Primary antibody: ab4729 (H3 acetyl K27)
    Dilution: 1/100

    ab4729 strongly stained histones of mouse ES cells. However, fluroescence was greatly diminished following pre-blocking using a H3 acetyl K9 peptide. This suggests the antibody cross-reacts with the K9 and K27 residues.

     

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Lanes 1 & 3 : Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 0.2 µg/ml
    Lanes 2 & 4 : Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 0.1 µg/ml

    Lanes 1-2 : Calf thymus histone lysate
    Lanes 3-4 : Calf thymus histone lysate with Human Histone H3 (acetyl K27) peptide (ab24404) at 2 µg

    Lysates/proteins at 1 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit (HRP) at 1/2000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    ab4729 specifically recognises acetyl K27 histone H3 in catlf thymus histone lysate, which is specifically blocked using the immunizing peptide ab24404.
  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)

    HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were incubated at 37°C for 6 hours with vehicle control (0 μM) and different concentrations of sodium butyrate (ab120948). Increased expression of histone H3 (acetyl K27)(ab4729) in HeLa cells correlates with an increase in sodium butyrate concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 2.5 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4927 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10,000 dilution and visualised using ECL development solution.

  • Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
    Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation, nuclear Lysate - Butyrate treated at 2.5 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds


     

     

Protocols

  • PCR primers used to test ab4729 in ChIP at Abcam

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1170)

Publishing research using ab4729? Please let us know so that we can cite the reference in this datasheet.

ab4729 has been referenced in 1170 publications.

  • Tambalo M  et al. Enhancer activation by FGF signalling during otic induction. Dev Biol 457:69-82 (2020). PubMed: 31539539
  • Tarumoto Y  et al. Salt-inducible kinase inhibition suppresses acute myeloid leukemia progression in vivo. Blood 135:56-70 (2020). PubMed: 31697837
  • Han M  et al. Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastoma. EMBO Mol Med 12:e10924 (2020). PubMed: 31777202
  • Castelijns B  et al. Hominin-specific regulatory elements selectively emerged in oligodendrocytes and are disrupted in autism patients. Nat Commun 11:301 (2020). PubMed: 31949148
  • Domingues AF  et al. Loss of Kat2a enhances transcriptional noise and depletes acute myeloid leukemia stem-like cells. Elife 9:N/A (2020). PubMed: 31985402
View all Publications for this product

Customer reviews and Q&As

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1-10 of 67 Abreviews

Flow Cytometry abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (Thymocytes)
Permeabilization
Yes - eBioscience™ Fixation/Permeabilization Concentrate
Gating Strategy
Thymocytes using CD45
Specification
Thymocytes
Preparation
Cell harvesting/tissue preparation method: Thymus was digested with Liberase in 1x PBS + 2% FCS
Sample buffer: 1x PBS + 2% FCS
Fixation
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 24 2021

Western blot abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 10 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 10 2020

ChIP abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - whole cell (Heart)
Negative control
Input
Specification
Heart
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
ENCODE H3K27ac ChIP-seq data (ENCSR699XHY)
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Abcam user community

Verified customer

Submitted Aug 06 2020

Western blot abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Platynereis dumerilii Tissue lysate - nuclear (Whole animals: 4 days & 7 months post fertilizatio)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
25 µg
Specification
Whole animals: 4 days & 7 months post fertilizatio
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. Quentin Schenkelaars

Verified customer

Submitted Jun 24 2020

ChIP abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - whole cell (0.03% digitonin in CUT&Tag wash buffer)
Specification
0.03% digitonin in CUT&Tag wash buffer
Detection step
Other
Type
Native ChIP (N-ChIP)
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Dr. Takayo Sasaki

Verified customer

Submitted Jun 05 2020

ChIP abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - other (mouse ES cells, 300,000 cells, digitonin 0.03% in)
Specification
mouse ES cells, 300,000 cells, digitonin 0.03% in
Detection step
Other
Type
Native ChIP (N-ChIP)
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Abcam user community

Verified customer

Submitted Feb 03 2020

Western blot abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary ear fibroblast)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
Primary ear fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jul 26 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh/, hepatocyte-derived carcinoma cell line)
Permeabilization
Yes - 0.5% TX100
Specification
Huh/, hepatocyte-derived carcinoma cell line
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
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Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Feb 20 2019

Western blot abreview for Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Schmidtea mediterranea Cell lysate - whole cell (Whole worm)
Gel Running Conditions
Reduced Denaturing (Invitrogen™ NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 10-well)
Loading amount
15 µg
Specification
Whole worm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Oct 22 2018

1-10 of 67 Abreviews

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