Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM156] to Histone H3 (acetyl K79)
- Suitable for: ICC/IF, WB, ELISA, ChIP
- Reacts with: a wide range of other species
Product nameAnti-Histone H3 (acetyl K79) antibody [RM156]
See all Histone H3 primary antibodies
DescriptionRabbit monoclonal [RM156] to Histone H3 (acetyl K79)
Specificityab214731 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.
Tested applicationsSuitable for: ICC/IF, WB, ELISA, ChIPmore details
Species reactivityReacts with: a wide range of other species
Synthetic peptide within Histone H3 (acetyl K79). The exact sequence is proprietary.
- Acid extracts from HeLa cells treated with sodium butyrate; HeLa whole cell lysate, treated with Sodium Butyrate; HeLa cells with Sodium Butyrate treatment.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.5 - 2 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 15 kDa.|
|ELISA||Use a concentration of 0.2 - 1 µg/ml.|
|ChIP||Use a concentration of 2 - 10 µg/ml.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H3 family.
Developmental stageExpressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
modificationsAcetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- H3 histone family member E pseudogene antibody
- H3 histone family, member A antibody
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ChIP performed on HeLa cells with or without
Sodium Butyrate treatment, using ab214731 at 5μg/mL dilution. Real-time PCR was
performed using primers specific to the gene
All lanes : Anti-Histone H3 (acetyl K79) antibody [RM156] (ab214731) at 1 µg/ml
Lane 1 : acid extracts from HeLa cells untreated
Lane 2 : acid extracts from HeLa cells treated with sodium butyrate
Predicted band size: 15 kDa
Immunofluorescent analysis of HeLa cells treated with
sodium butyrat labeling Histone H3 (Acetyl-Lys79)
with ab214731 at 2 μg/mL dilution (red); actin filaments have
been labeled with fluorescein phalloidin (green).
Sandwich ELISA labeling acetylated Histone H3
at Lys 79 in HeLa whole cell lysate, treated or
untreated with Sodium Butyrate using ab214731 at 5 μg/mL dilution as the capture antibody and
biotinylated anti-H3CT at 1 μg/mL dilution as the
ab214731 specifically reacts to Histone H3 acetylated
at Lysine 79 (K79ac). No cross reactivity with acetyl
ated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14
(K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), or Lysine 122 (K122) in Histone H3.
ab214731 has not yet been referenced specifically in any publications.