Product nameAnti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade
See all Histone H3 primary antibodies
DescriptionRabbit polyclonal to Histone H3 (acetyl K9, phospho S10) - ChIP Grade
Tested applicationsSuitable for: ICC/IF, ChIP, WBmore details
Species reactivityReacts with: Mouse, Chicken, Human, Drosophila melanogaster
Predicted to work with: Rat, Cow, Xenopus laevis, Caenorhabditis elegans, a wide range of other species
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
- interphase HepG2 cells (see Abreview)
General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography. The resulting IgG fraction is further purified by absorption on the unmodified histone H3 peptide (human, amino acids 7-20).
ChIP Related Products
Our Abpromise guarantee covers the use of ab12181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 17675409|
|WB||1/1000. Detects a band of approximately 17 kDa.|
RelevanceHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination, are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by the modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Phosphorylation, particularly that of histones H1 and H3, has long been implicated in chromosome condensation during mitosis. However, converging evidence suggests that H3 phosphorylation (specifically serine 10) is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. The potential importance of the serine 10 phosphorylation mark in H3 is strengthened by the finding that MSK1, a kinase activated by growth factor and stress stimuli, also phosphorylates H3 in vitro. H3 phosphorylation at serine 10 in conjunction with phosphorylation at serine 28, is also required for proper segregation and condensation of chromosomes during mitosis and meiosis.
- H3 3 like sequence MH921 antibody
- H3 3A antibody
- H3 histone antibody
This image was kindly supplied as part of the review submitted by Monika Harrer.
All lanes : Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade (ab12181)
Lane 1 : Whole cell extract of NIH3T3 cells
Lane 2 : Whole cell extract of NIH3T3 cells with [Ac-Lys9, pSer10] Histone H3 peptide
Lane 3 : Whole cell extract of NIH3T3 cells with [non-Ac, non-phos] Histone H3 peptide
Lane 4 : Whole cell extract of NIH3T3 cells with [Ac-Lys9] Histone H3 peptide
Lane 5 : Whole cell extract of NIH3T3 cells with [pSer10] Histone H3 peptide
All lanes : Alkaline phosphatase conjugated Goat anti-Rabbit developed with a colorimetric substrate
Observed band size: 17 kDa why is the actual band size different from the predicted?
Inhibition is shown in Lane 2.
This product has been referenced in:
- Carter SD et al. Acute Stress Enhances Epigenetic Modifications But Does Not Affect the Constitutive Binding of pCREB to Immediate-Early Gene Promoters in the Rat Hippocampus. Front Mol Neurosci 10:416 (2017). ChIP . Read more (PubMed: 29311809) »
- Wiersma M et al. Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes. Epigenetics Chromatin 9:52 (2016). ChIP ; Human . Read more (PubMed: 27895715) »