Key features and details
- Assay type: Cell-based (quantitative)
- Platform: Microplate reader
- Assay time: 5 hr
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 1 ng
Product nameHistone H3 Acetylation Assay Kit
See all Histone H3 acetylation kits
Sample typeTissue, Adherent cells, Suspension cells
Assay typeCell-based (quantitative)
Range1 ng - 100 ng
Assay time5h 00m
Species reactivityReacts with: Mouse, Human
Predicted to work with: Mammals
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.
Histone H3 Acetylation Assay Kit (ab115102) allows the user to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 10X Lysis Buffer 1 x 5ml 1 x 10ml 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Acetylated Histone H3 Control (60 µg/mL) 1 x 10µl 1 x 20µl Antibody Buffer 1 x 6ml 1 x 12ml Blocking Buffer 1 x 10ml 1 x 20ml Capture Antibody, 100 µg/mL 1 x 25µl 1 x 50µl Detection Antibody, 400 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 5ml 1 x 10ml Extraction Buffer 1 x 8ml 1 x 16ml Histone Buffer 1 x 0.5ml 1 x 1ml Stop Solution 1 x 3ml 1 x 6ml
ab115102 has been referenced in 3 publications.
- Schwarz A et al. Induction of Regulatory T Cells and Correction of Cytokine Disbalance by Short-Chain Fatty Acids: Implications for Psoriasis Therapy. J Invest Dermatol 141:95-104.e2 (2021). PubMed: 32544478
- Chen CL et al. Arginine is an epigenetic regulator targeting TEAD4 to modulate OXPHOS in prostate cancer cells. Nat Commun 12:2398 (2021). PubMed: 33893278
- Svoboda P et al. Nuclear transport of nicotinamide phosphoribosyltransferase is cell cycle-dependent in mammalian cells, and its inhibition slows cell growth. J Biol Chem 294:8676-8689 (2019). PubMed: 30975903