Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/30000

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
This lot gives an exceptional dilution factor ( yes, 1/30K) ab1791 was tested at the same time and gave the same positivity in a wide range of species, under identical conditions.

Mr. Carl Hobbs

Verified customer

Submitted Apr 20 2015

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