Western blot abreview for Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Recombinant protein (Recombinant octamer & purified K562 cell histone)
Gel Running Conditions
Reduced Denaturing (18%)
Loading amount
0.5 µg
Specification
Recombinant octamer & purified K562 cell histone
Blocking step
Odyssey blocking solution 1/1 (vol/vol) PBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Odyssey blocking solution 1/1 (vol/vol)
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-rabbit IRDYE800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Odyssey Imaging channel 800
Negative control
Other histones.
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
Positive control
Histone H3 from Drosophila and Human, recombinant and native.

Additional data

Additional Notes
Lane 1 K-562 octamer 1 microgram, Lane 2 K-562 octamer 500 nanograms, Lane 3 Recombinant Human octamer 500 nanograms, Lane 4 Recombinant Drosophila octamer 500 nanograms.

Dr. Ragnhild Eskeland

Verified customer

Submitted May 11 2015

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