Antibody worked really well. The IP was performed under ChIP conditions. in brief, 1ug of antibody to 1A260 of the nuclear lysate was used and incubated for 16hrs at 4 degrees and the matrix was added afterwards and incubated for 3hrs at 4 degrees with rotation. Western blot was run rather than qPCR. ID=immunodepleted.
Submitted Feb 28 2017
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