Immunoprecipitation abreview for Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control and ChIP Grade

Good
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (MDA-MB-231 cells)
Total protein in input
1e+007 cells
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
MDA-MB-231 cells

Other product details

Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C

Western Blot antibody

Dilution
1/6000
Name
Abcam antibody:(not specified yet)

Additional data

Additional Notes
Antibody worked really well. The IP was performed under ChIP conditions. in brief, 1ug of antibody to 1A260 of the nuclear lysate was used and incubated for 16hrs at 4 degrees and the matrix was added afterwards and incubated for 3hrs at 4 degrees with rotation. Western blot was run rather than qPCR. ID=immunodepleted.

Ifeoluwa Adewumi

Verified customer

Submitted Feb 28 2017

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