Western blot abreview for Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Carp Tissue lysate - nuclear (Brain tissue from crucian carp (Carassius carassiu)
Gel Running Conditions
Reduced Denaturing (4-15 % gradient gel)
Loading amount
10 µg
Specification
Brain tissue from crucian carp (Carassius carassiu
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 21°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Same as blocking buffer
Dilution
1/10000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-rabbit IRDYE 800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Odyssey Imaging channel 800
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa

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Submitted May 23 2018

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