Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
5 µg
Specification
HEK293
Treatment
50nM TSA for 24hrs
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/5000
Detection
Detection method
Odissey
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
Additional data
Additional Notes
lane1 HEK293 cells untreated
lane2 HEK293 cells treated with 50nM TSA for 24h
lane2 HEK293 cells treated with 50nM TSA for 24h
Abcam user community
Verified customer
Submitted Jul 22 2010