Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cells)
Specification
Hela cells
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C

Other product details

Dilution
1/300
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 0.2 % Fish scale gelatin in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Histone H3 is clearly visualized (green channel) in the nuclear of Hela cells by immunostaining using Histone H3 antibody - ChIP Grade (ab1791)

Abcam user community

Verified customer

Submitted Oct 04 2010

Sign up