Western blot abreview for Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (Mitochondrial and nclear fraction from skeletal mu)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Mitochondrial and nclear fraction from skeletal mu
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk (TBS-tween)
Dilution
1/1000

Secondary antibody

Dilution
1/4000
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
6 minute(s) and 39 second(s)
Bands
Specific: 17 kDa

Additional data

Additional Notes
As expected HH3 band came up in the nuclear fraction only.

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Submitted Mar 16 2016

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