Western blot abreview for Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (HT1080)
Gel Running Conditions
Non-reduced Denaturing (18%)
Loading amount
5 µg
Specification
HT1080
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS BSA1%
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa

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Submitted Jan 09 2018

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