Western blot abreview for Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Schmidtea mediterranea Tissue lysate - whole (Whole worm lysate)
Gel Running Conditions
Reduced Denaturing (4-12%BT-gel)
Loading amount
10 µg
Specification
Whole worm lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/10000

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 15KDA kDa
Positive control
HELA NUCLEAR EXTRACT

Additional data

Additional Notes
First Lane HELA - Second Lane S. Mediterranean whole worm lysate Bands appear to be around 14-16KDA. Non-specific bands are observed in both.

Anish Dattani

Verified customer

Submitted Feb 22 2018

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