Histone H3 antibody was used as a control to measure the relative fold occupancy for the H3K4me1 and H3K27ac on enhancer and a nearby control region.
ChIP Dilution buffer: 50mM Tris (pH7.4), 10 mM HEPES-KOH (pH7.5), 100 mM NaCl, 1 mM EDTA pH8, 0.5% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS.
2 microliter of antibody used per ChIP
Overnight incubation with Protein A/G beads, 4h after addition of antibody.
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Submitted May 08 2018
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