Western blot abreview for Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-17%)
Loading amount
10 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Other product details

Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/2500
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa

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Submitted May 28 2018

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