Western blot abreview for Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Cos7)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
Cos7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TTBS
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab (from
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
1 second(s)
Bands
Specific: 15 kDa Non-specific: 17 kDa

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Submitted Aug 22 2018

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