Western blot abreview for Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (MEF-Acid extracted histones)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
2 µg
Specification
MEF-Acid extracted histones
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% milk TBST
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 17 kDa Non-specific: none kDa

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Submitted Oct 11 2018

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