Flow Cytometry abreview for Anti-Histone H3 antibody - ChIP Grade

Good
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (Embryonic stem cells)
Specification
Embryonic stem cells
Preparation
Cell harvesting/tissue preparation method: ES cell colonies were trypsinized and strained through cell strainer
Sample buffer: cells were kept in FACS buffer or PBS
Fixation
Commercial Fixation concentrate
Permeabilization
Yes - Commercial Permeabilization Buffer
Gating Strategy
all living cells were gated

Other product details

Concentration
1 µg/cells
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Permeabilization Buffer

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit-PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/500

Ms. Nadine Obier

Verified customer

Submitted Aug 21 2007

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